rss_2.0Bulletin of Medical Sciences FeedSciendo RSS Feed for Bulletin of Medical Scienceshttps://sciendo.com/journal/ORVTUDERThttps://www.sciendo.comBulletin of Medical Sciences 's Cover200300Investigation of the phytochemical and antimicrobial activity of Primula veris L. extractshttps://sciendo.com/article/10.2478/orvtudert-2020-0013<abstract> <title style='display:none'>Abstract</title> <p>Methanol, ethanol (50%) and aqueous extracts were prepared from the roots, leaves and flowers of Primula veris collected and dried under natural conditions in meadows near Marosludas in April 2018. The polyphenol contents of the plant parts, as well as the antioxidant capacity were measured by ABTS spectrophotometric methods. For the microbiology section, aqueous and ethanolic extracts (100 mg/ml) were prepared from the roots, leaves and flowers of Primula veris followed by testing of their antimicrobial activity against human pathogenic yeasts (Candida albicans, Candida parapsilosis, Candida glabrata) and bacteria (Gram negatives:, Escherichia coli, Acinetobacter sp., Pseudomonas aeruginosa, Enterobacter cloacae; Gram-positive: Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus) by agar well diffusion method.</p> <p>Ethanol proved to be the most effective solvent for all three drugs to determine the polyphenol content. The flower drug contains the highest amounts of polyphenols. With the ABTS method of antioxidant measurement, ethanolic extracts are more effective than aqueous extracts.</p> <p>Agar well diffusion studies showed that all extracts caused complete inhibition within the inhibition zone in the yeasts tested. The inhibitory effect of aqueous extracts was stronger than that of ethanolic extracts in the case of all three plant parts. The greatest inhibition was caused by extracts of the roots with ethanol. The three yeasts tested showed similarly high sensitivity to roots extracts, while the inhibitory effect was weaker for leaf extracts. Candida parapsilosis was resistant to leaf and flower extracts. Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus, like fungi, showed good sensitivity to extracts, while other bacteria showed little or no inhibitory effect.</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00Incidence of multidrug-resistant bacteria in tracheal secretions from critically ill patients in the intensive care unithttps://sciendo.com/article/10.2478/orvtudert-2020-0017<abstract> <title style='display:none'>Abstract</title> <p>Ventilator-associated pneumonia is a severe nosocomial infection that affects the disease course of critically ill patients. Awareness of potential pathogens is essential for prevention, early detection, and proper treatment, as well.</p> <p>In this retrospective cross-sectional study, we investigated the tracheal secretions collected from critically ill patients with the aim to detect the occurrence of multidrug-resistant bacteria. We examined the bacteriological culture results of the tracheal secretions of the patients hospitalized at the Intensive Care Unit of Tîrgu Mureș Emergency Clinical County Hospital between 1st November 2017 and 31st January 2018. Admission diagnoses and comorbidities were recorded, and white blood cell counts were monitored. We determined the quality of the lower respiratory samples by microscopic examination and the results of the microbiological tests, taking into account the germ count of pathogens and the antibiotic-resistance pheno-type. During the three months, 194 samples were received from 107 patients for bacteriological examination. After the first sample collection 34 (31.77%) tracheal secretions were positive for pathogens, while in the remaining samples normal upper respiratory bacterial flora was found. From the 34 positive samples, 22 were colonizing pathogens and 30 were isolated in a clinically significant amount. Predominantly Staphylococcus aureus (n=14; 26.9%), Klebsiella pneumoniae (n=9; 17.3%), Escherichia coli (n=8.1%) and other Gram-negative bacteria (n=21; 40.4%) were identified. Among these strains 38 (73.07%) were not multidrug-resistant. The rate of positivity of individual sampling showed a positive correlation with the average duration of hospital stay (p=0.0016; r=0.8740). A total of 26 patients developed early-onset or late-onset ventilator-associated pneumonia. Potential risk factors for infection with multidrug-resistant bacteria were found. We can conclude that recently admitted patients in the intensive care unit are rarely carriers of multidrug-resistant bacteria, but become colonized or infected with multidrug-resistant strains during long-term intensive care.</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00Study of pharmaceutical competences among the Romanian population, based on an online surveyhttps://sciendo.com/article/10.2478/orvtudert-2020-0009<abstract> <title style='display:none'>Abstract</title> <p>From the year 1990s the pharmaceutical competences<ext-link ext-link-type="uri" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="https://www.thesaurus.com/browse/assignment">https://www.thesaurus.com/browse/assignment</ext-link> based on drug supply has been completed with the patient consultation and communication. This fact has greatly expanded the work of the pharmacists. As a result of this, the concept of pharmaceutical care has been introduced and normalized in several countries. But, in spite of all efforts there are still major differences in health care policies and practices among European countries. The aim of this study was to check the estimation of the pharmacists’ duties by patients and the attitude towards online medicine acquisition. Materials and methods: we used a four - part questionnaire created in Google Forms. The content of the questionnaire consisted of: a.) checking of the demographic data, b.) assessment of patients’ attitudes towards pharmacies, c.) assessment of pharmacist competences, d.) examining the viability of an online drug supply. Results and discussion: 524 persons completed the questionnaire (88.7% women, 11.3% men). 66.8% were from the city and 33.2% from the village, respectively. Patients’ adherence to pharmacies in most cases depended on the location of the pharmacy (65.1%) and the kindness (36.5%) and competence (36.5%) of the pharmacist. Checked with three parallel questions, it turned out that the number of questions occurring in the patients’ mind at home always exceeded the proportion of the answered questions in the pharmacy. 68.1% of the respondents consulted a pharmacist in the absence of a physician. Many (54.4%) of that surveyed searched healthcare information on Google search, but few of them ordered medicinal products via Internet. Based on the results of our survey, 18.3% of the respondents searched healthcare information on healthcare professionals’ online platform. Their trust was much greater in such platforms than in information found on Google search. Conclusion: A slight loyalty to a certain pharmacy can make it difficult to follow the drug therapy. This adherence to a pharmacy can be increased by the kindness and skills of the pharmacist. Although patients search healthcare information on online interfaces this information do not convince them to order medicinal products via Internet. The high degree of trust in pharmacist or physician managed homepages highlight the importance of these interfaces.</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00Determination of enantiomeric purity of esomeprazole by capillary electrophoresishttps://sciendo.com/article/10.2478/orvtudert-2020-0016<abstract> <title style='display:none'>Abstract</title> <p>Proton pump inhibitors are the most effective agents used in gastric hyper-acidity-related disorders. Omeprazole is a benzimidazole-derivative compound with an asymmetric sulphur in its structure, which generates its chiral character.</p> <p>Esomeprazole (S-omeprazole) was the first proton pump inhibitor introduced as an enantiomerically pure compound in therapy, after the successful chiral switch of the racemic omeprazole.</p> <p>This work is aimed at performing a complementary study to an already published chiral separation method of omeprazole. As preliminary analysis, the electrophoretic behavior of omeprazole enantiomers and the possible mechanism of the chiral resolution was studied using different background electrolytes containing different β-cyclodextrin derivatives, as chiral selectors. The robustness of the chiral separation method was tested by applying a Plackett-Burman design. The method was validated according to current ICH guidelines and proved to be reliable, linear, precise and accurate for the determination of 0.2% R-omeprazole as chiral impurity in esomeprazole samples. The validated method was successfully used for the analysis of esomeprazole-containing gastro-resistant tablets.</p> <p>According to our results, valuable information on the mechanism of chiral separation of omeprazole was gained and the application area of the previously developed method was successfully enlarged. The presented rapid and cost-effective capillary electrophoresis method proved to be suitable for the determination of enantiomeric purity of esomeprazole from pharmaceutical preparations and could represent an alternative for the available compendial methods.</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00Mapping of Echinacea-based food supplements on the Romania market and qualitative evaluation of the most commonly used productshttps://sciendo.com/article/10.2478/orvtudert-2020-0008<abstract> <title style='display:none'>Abstract</title> <p>The aim of the study was to explore dietary supplements containing Echinacea on the Romanian market and their qualitative characterization.</p> <p>The products available on the market were aggregated in 2018, through an electronic search based on the register of the Romanian Medicine Agency (Agenția Națională a Medicamentului şi a Dispozitivelor Medicale din România – ANMDMR) and the list of dietary supplements registered by the Ministry of Agriculture and Rural Development (Institutul Național de Cercetare-Dezvoltare pentru Bioresurse Alimentare – IBA București, Serviciul național pentru plante medicinale, aromatice și produse ale stupului). There are no Echinacea containing medicines registered in Romania. However, there are 58 dietary supplements in the register, 52% of which are mono-components, 29% contain other herbs, plant extract or vitamins, while 19% are registered as tea. The label of 80% of monocomponent products and 76% of multicomponent supplements contains insufficient information: the plant name, its used part and processing methods (grist, extract, quantity) are not clearly identified. Among the listed dietary supplements, the 12 most commonly used formulations in pharmaceutical practice were subjected to phytochemical chromatographic evaluation: TLC and/or HPLC analysis were used. Three of seven monocomponent products showed proper chromatographic fingerprint, by TLC analysis. One monocomponent sample did not have an adequate chromatographic fingerprint. The labelling of multicomponent products was not appropriate. The TLC test suggests that based on the resulting fingerprint they contain E. purpureae herba. However, due to the presence of other components, the TLC does not allow a clear conclusion regarding the exact composition of the products.</p> <p>The developed HPLC method enables quantification of the concentration of caffeic acid, chicoric acid, echinacoside, chlorogenic and caftaric acids mixture in dietary supplements. None of the tested products contained echinacoside, which is a specific component of E. angustifolia and E. pallida root. In our method, the quantification of caftaric acid is approximate, because it partially overlaps the chlorogenic acid, which is a common component of plant samples, but negligible in Echinacea sp. The tested dietary supplements have a caffeic acid content of 20-140 µg/g, a chicoric acid content of 0.19-2.64 mg/g; the mixture of chlorogenic and caftartic acid is about 0.23-2.07 mg/g.</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00Improving the Management of Pediatric Upper Urinary Tract Infections at Odorheiu Secuiesc Hospitalhttps://sciendo.com/article/10.2478/orvtudert-2020-0015<abstract> <title style='display:none'>Abstract</title> <p>The primary goal of our retrospective analysis was to check the correctness of the diagnosis, and to identify the potential diagnostic errors among patients coded with N10 diagnosis in 2009, 2014 and 2019 in the pediatric ward of Odorheiu Secuiesc Hospital; we also assessed protocol-adherence in treatment and prophylaxis, and changes in antibiotic resistance of the isolated E. coli strains.</p> <p>During this 10 year-period significant improvements have been achieved in the treatment of upper urinary tract infections: in 2009 the diagnosis could be disputed in 31 out of 61 cases, while in 2019 this ratio is only 2 out of 31 cases; the number of cases pretreated with antibiotics decreased to a third, contaminated urine samples are rare to occur, the unmotivated antibiotics prophylaxis is essentially nonexistent, and there are no major documentation deficiencies either.</p> <p>In our study we present the antibiotic susceptibility of 50 E. coli strains isolated from the study group, which suggests some favorable changes.</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00The Effect of Deep Brain Stimulation on High Frequency Oscillations in a Chronic Epilepsy Modelhttps://sciendo.com/article/10.2478/orvtudert-2020-0014<abstract> <title style='display:none'>Abstract</title> <p>Temporal lobe epilepsy (TLE) is a severe neurological disease which is often pharmacoresistant. Deep brain stimulation (DBS) is a novel method for treating epilepsy; however, its mechanism of action is not fully understood. We aimed to study the effect of amygdala DBS in the pilocarpine model of TLE. Status epilepticus was induced by pilocarpine in male Wistar rats, and spontaneous seizures occurred after a latency period. A stimulating electrode was inserted into the left basolateral amygdala and two recording electrodes into the left and right hippocampus. A stimulus package consisted of 0.1 ms-long biphasic pulses applied regularly at 4 Hz for 50 seconds. This package was repeated four times a day, with 5-minute pauses, for 10 days. We also used an age-matched healthy control group of stimulated animals and another one of sham-operated rats. From the hippocampal local field potentials high frequency oscillations (HFOs) were analyzed as these are promising epilepsy biomarkers. HFOs are short oscillatory events between 80-600 Hz which were detected offline using an open-source application of MATLAB, the RIPPLELAB system. We found that the HFO rate was significantly higher in pilocarpine-treated rats compared to the control groups (0.41 ± 0.14 HFO/min vs. 0.006 ± 0.003 in the stimulated control group and no HFO in the sham-operated group). In the pilocarpine group an instantaneous decrease in HFO rate was observed while the stimulation was on (0.44 ± 0.15 HFO/min vs 0.07 ± 0.03 HFO/min, p=0.017). The effect was short-lived because the frequency of HFOs did not change significantly in the time windows between stimulus packages or during the ten-day stimulation period.</p> <p>The difference of HFO rates between epileptic and control groups could be used in the electrographic assessment of epilepsy. The decreased frequency of HFOs during stimulation may be useful to study the efficacy of DBS.</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00Non-surgical therapy of diabetic foot infections based on recent guidelines and published datahttps://sciendo.com/article/10.2478/orvtudert-2020-0006<abstract> <title style='display:none'>Abstract</title> <p>Chronic, non-communicable diseases are affecting the lives of more and more people worldwide; due to obesity and inadequate eating habits, the proportion of diabetics is on an increasing trend globally. The development of a number of macro- and microangiopathic pathologies associated with the onset of diabetes affects the life expectancy of patients. Diabetic foot infection (DFI) is one of the most serious long-term complications of diabetes and may be considered an intersection of infectious and non-infectious diseases; these infections can significantly reduce the quality of life of affected patients. The purpose of this review was to summarize the latest recommendations for conservative (non-surgical) therapy for DFIs and diabetic foot osteomyelitis (DFO).</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00Headache with cranial nerve palsy – a diagnostic and therapeutic challenge in three caseshttps://sciendo.com/article/10.2478/orvtudert-2020-0012<abstract> <title style='display:none'>Abstract</title> <p>Headaches are among the most common complaints in the Neurology Clinic. As a symptom, headache is often a problem not only for the patient, but also for the doctor, as in addition to primary headaches, secondary headaches can have various etiologies. Among the primary headaches, migraine and cluster headaches may present cranial nerve symptoms. The differential diagnosis of secondary headaches can be difficult. We have to include vascular, neoplastic, infectious, inflammatory, toxic, autoimmune causes, where diagnosis requires complex laboratory and/or imaging studies, while the absence of abnormal findings often suggests autoimmune or unknown origin. In our review, we aim to present three cases where the headache was associated with cranial nerve involvement, mainly ophthalmoparesis. The course of the disease was long in all three cases, characterized by symptom-free periods alternating with paroxysmal headaches and diverse cranial nerve palsies. Most frequently, the oculomotor and abducent cranial nerve palsies were observed, but the involvement of trigeminal and facial nerves occurred as well. Based on the symptoms, there was a suspicion of a lesion of the cavernous sinus in all three cases, however, this couldn’t be confirmed by advanced neuroimaging techniques. In addition to symptomatic treatment, steroid therapy improved the complaints, but neurological signs did not disappear in all cases. Furthermore, the signs reappeared after a short time or a different cranial nerve was involved. The lack of a positive diagnosis, the fluctuating course of the disease, and the side effects of steroid therapy demand a long-term follow-up, which is possible only through an appropriate doctor-patient relationship. As guidelines are difficult to elaborate on for such cases, continuous re-evaluation of diagnosis and treatment are required.</p> </abstract>ARTICLE2021-03-30T00:00:00.000+00:00Chemotherapy induced liver toxicity in children with malignant diseaseshttps://sciendo.com/article/10.2478/orvtudert-2018-0002<abstract><title style='display:none'>Abstract</title><p>A broad spectrum of chemotherapy is being used in the therapy of childhood cancers, which may induce liver injury, impairing quality of life and efficacy of the treatment. History of, especially viral, liver diseases may increase toxicity. The aim of the paper is to assess the incidence, type and grade, predisposing factors and treatment options of drug-induced liver injury in children with malignant diseases under cytostatic therapy at the Hemato-Oncology Department of the Pediatric Clinic 2 from Targu-Mures, over a time period spanning from 2012 to 2017. The results of the study may serve as a foundation for such treatment strategies which would enable optimal outcomes with fewer cases of liver toxicity.</p><p>During this period, we treated 26 patients with acute lymphoblastic leukemia (ALL), two patients with acute myeloblastic leukemia (AML), one patient with lymphoma and seven with solid tumors. We found liver toxicity in 77% of the patients treated for ALL, mainly during the maintenance therapy (65%) with oral 6-mercaptopurine and methotrexate. The most common clinical signs were anorexia, nausea, vomiting, abdominal pain and faltering weight gain. Cholestasis developed in two patients, while hepatocytolysis was the most common observed event (n = 24). Liver fibrosis, hypersplenism, portal hypertension and esophageal varices were found in two patients. One patient required endoscopic ligation of esophageal varices. Elevation of serum bilirubin appeared in two patients, while hypoproteinemia was observed in nine patients. None of the patients developed acute liver failure. We treated liver toxicity with hydration, alkalinization, i.v. Aspatofort, Aminosteril-N Hepa 8%, oral acetylcysteine, silymarin, ursodeoxycholic acid, Liv-52, Sargenor, and Essentiale forte.</p><p>We found hepatotoxicity in 77% of the ALL patients undergoing chemotherapy, similar results have been published by other authors.</p><p>Hepatotoxicity may develop through direct hepatic effects of cytostatics, or a preexisting liver disease impairs the metabolism and excretion of the drug, increasing its toxic effects. In our patients hepatotoxicity can be explained mainly by direct liver-injury, previous infections with hepatotropic viruses, such as cytomegalovirus, were detected only in three patients.</p><p>Liver injury appeared in 77% of our ALL patients; 65% occurred during maintenance therapy with oral 6-mercaptopurine and methotrexate. Close followup of liver function during chemotherapy is mandatory for optimal results.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00Current aspects of polycystic ovary syndrome I: definition, pathophysiology, clinical manifestations, diagnosis and complicationshttps://sciendo.com/article/10.2478/orvtudert-2018-0011<abstract><title style='display:none'>Abstract</title><p>Polycystic ovary syndrome (PCOS) is the most frequent endocrine disease among women with childbearing potential, the best-known cause of hirsutism, with a hypothesized prevalence of 8-22%. The first part of the paper discusses the conceptional evolution of the syndrome, from its description in 1935 by Stein and Leventhal till today. It describes the changes in the criteria systems, emphasizing that the Rotterdam criteria, proposed in 2003 by the European Society for Human Reproduction and Embryology/American Society for Reproductive Medicine, are still valid today. This system basically differs from earlier (1990) NIH-criteria in one aspect: it introduced two newer phenotypes, one without hyperandrogenism and the other with ovulatory cycles, so it distinguishes 4 phenotypes. The etiology and pathogenesis of PCOS is heterogeneous, multifactorial, poorly understood. We present the 3 leading hypotheses (1 - hypothalamo-hypophyseal disturbances, 2 – primary enzyme disorders in ovarian, or ovarian/adrenal steroidogenesis, resulting primarily in hyperactivity of 17alpha-hydroxylase/17,20-lyase, 3 – insulin resistance-hyperinsulinism and other metabolic dysfunctions). We emphasize the role of genetically determined hyperandrogenism, that of insulin resistance-hyperinsulinism and the importance of reinforcing each other. Subsequently, the aggravating aspects of the frequently associated metabolic syndrome are discussed, and then the effects of the mentioned pathological processes on the endocrine and other organ structures participating in the regulation of sexual functions. We stress the hypothetical role of perinatal and pubertal androgen exposition in the pathogenesis of PCOS. The mechanisms of anovulation and those of the endometrial lesions are discussed, too. The clinical manifestations, the paraclinical and laboratory examinations, the positive and differential diagnosis and the complications are also presented. We intend to deal with the therapeutic aspects of PCOS in an upcoming paper.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00Commemoration of Kárpáti Gizella, the first woman who took her degree in medical science in Kolozsvár at Ferenc József Universityhttps://sciendo.com/article/10.2478/orvtudert-2018-0001<abstract><title style='display:none'>Abstract</title><p>On 31<sup>st</sup> of December 1895 Gyula Wlassics (1852-1937), minister of religion and education, published his Decree No 72 039, which allowed women to study medical science, pharmacy and humanities at universities. In Kolozsvár Gizella Kárpáti was the first woman who registered at the Faculty of Medicine of Ferenc József University.</p><p>Gizella Kárpáti was born in Kolozsvár on 3rd August 1884. After finishing secondary school in June 1902, in the autumn of the same year she registered at the Faculty of Medicine. The marks she got at the university examinations in the following 10 semesters proved that she could cope with her task. In the years spent at the university she met medical student József Szabó (born in 1882) whom she married later. They both started their medical career at Károly Lechner’s Neuro-Psychiatry Institute. From 1909 Gizella Kárpáti was a payed assistant, from 1911 she became assistant lecturer. Her husband became an assistant lecturer from the year 1908, then he was appointed lecturer and then honorary lecturer. Meanwhile he had to go to the front where he was followed by his wife. In 1919, after the professors of the university refused the act of swearing the oath of allegiance, they both made their escape from Kolozsvár together with the greater part of their fellow professors. After a short time they settled in Szeged, where József Szabó was appointed university professor. Here he published his book „Elmekórtan” in 1925. In the 1927-28 academic year he became dean, then vice-dean of the Faculty of Medicine, but in the year 1929 he died unexpectedly. In Szeged, Gizella Kárpáti being engaged in bringing up her two children, did not work as a physician. One of her children, Miklós, continued his parents’ profession, and became a physician. Gizella and her children returned to Kolozsvár for a short time, between 1940-1944, then they moved to Budapest and finally they settled in Szeged again. In 1929 József Szabó and in 1953 Gizella Kárpáti were entombed in one of the graves of honour of the Szeged central cemetery.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00The prognostic role of thrombophilia in the treatment of infertilityhttps://sciendo.com/article/10.2478/orvtudert-2018-0004<abstract><title style='display:none'>Abstract</title><p>Thrombophilia refers to a coagulation disorder that predisposes to thrombosis and thus increases the risk of thrombotic events. Both inherited and acquired thrombophilia are associated with vascular thrombosis and pregnancy-related complications, including infertility, recurrent miscarriage, and premature birth.</p><p>Recently, thrombophilia has been increasingly encountered as an infertility factor, which gives the clinical relevance of the disease.</p><p>The aim of the study was to investigate the prognostic role of thrombophilia in the treatment of infertility and the pregnancy of thrombophilic women during assisted reproduction procedures.</p><p>Frequency of abortions increases and effectiveness of in vitro fertilization (IVF) decreases with age. Normal weight has a positive effect on assisted reproduction techniques (ART’s) outcome. Repeat IVF failure is more common in thrombophilia than in healthy women, and the “take home baby” ratio for IVF is 24%. Spontaneous abortion was most commonly observed in the PAI homozygous group followed by MTHFR homozygous mutation, MTHFR heterozygous mutation, and Factor V (Leiden) mutation. The most effective treatment was concomitant therapy with low molecular weight heparin and aspirin.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00Analysis of regional heterogeneities of the blood-brain barrier in humans and micehttps://sciendo.com/article/10.2478/orvtudert-2018-0005<abstract><title style='display:none'>Abstract</title><p>Proper functioning of the nervous system is largely dependent on the precise regulation of the neuronal environment. By shielding the central nervous system (CNS) from potentially harmful substances, the blood-brain barrier (BBB) has an indispensable role in this process. The BBB is a specialized system of endothelial cells lining brain microvessels, which – supported by pericytes and glial cells – form a selective barrier between the blood and the neural tissue. Under abnormal conditions, permeability of the BBB may increase, which may either trigger or aggravate the disease. Since CNS disorders – at least in their initial phase – usually do not involve the whole brain and spinal cord, but are localized to a certain region, our aim was to understand whether the BBB is regionally heterogeneous at the molecular level. By using bioinformatics tools, we analyzed expression levels of genes specific to cerebral endothelial cells, pericytes or astrocytes in different brain territories. Our results revealed regional heterogeneities in the expression of BBB-associated genes in both human and mouse. Expression pattern of efflux transporters – which have a major role in blocking passage of therapeutic agents through the BBB – proved to be diverse both among brain regions and between mouse and human. Our results indicate that: (1) <italic>in silico</italic> database analyses are suitable for group-based studies on gene functions, overcoming the limitations of single-gene analyses; (2) high-throughput tests should always be validated using other methods; (3) when using animal models, inter-species differences have to be always considered; (4) when comparing different brain regions, the BBB is heterogeneous at the molecular level, and this might have clinical significance.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00Famous hungarian chemists and pharmacists – modern chemistry founders. Part I. 1.2. Lajos Winklerhttps://sciendo.com/article/10.2478/orvtudert-2018-0013<abstract><title style='display:none'>Abstract</title><p>Pharmacist Winkler Lajos, PhD (1863-1939, born in Arad), professor at the University of Sciences from Budapest and member of the Hungarian Academy of Sciences, is considered the founder of modern analytical chemistry and drug analysis in Hungary.</p><p>He has developed and perfected a series of methods of volumetric and gravimetric analysis. Its original method, developed in 1888 for the determination of dissolved oxygen in water, is still used today.</p><p>Winkler Lajos also played an important role in the development of pharmaceutical education in Hungary</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00Use of internet as source of medical information and its impact among young Transylvanian Hungarian people - a comparative studyhttps://sciendo.com/article/10.2478/orvtudert-2018-0008<abstract><title style='display:none'>Abstract</title><p>The internet is the most popular information source in our digital world. Studies confirm that numerous people are using the internet to look up health-related information. There is no information about this trend among the Transylvanian Hungarian population. Our purpose was to assess the role of the internet in answering health-related problems for young Hungarian Transylvanian people, and its impact.</p><p>The participants (N=351) completed an online questionnaire with 28 items, which was available on Facebook, on the first page indicating their consent to a voluntary and anonymous survey. Our target was the generation below the age of 45. Descriptive, then comparative analysis was performed, based on gender and region of origin.</p><p>95.3% of the participants used the internet for finding health-related information, diagnosis, treatment or diet, without significant difference between subgroups, 70% at least once a month. Only 3.3% were instructed by their physician about the websites that provide health information, while 90% would require it. At least 64% of the respondents makes self-diagnosis at least sometimes, women more often, and nearly 25% frequently or always check the doctor’s opinion and/or the recommended treatment online. 40% of cases consider that their self-diagnosis was often the same as the physician’s final diagnosis, but only 33,2% agreed totally with their doctor. 47,4% of them were scared and/or became worried because of the information from the internet, especially women.</p><p>Based on the above, it is clear that online health information overtakes the traditional doctor-centered health information and makes it necessary for us to change our perspective of digital healthcare.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00Burnout syndrome among ICU personnelhttps://sciendo.com/article/10.2478/orvtudert-2018-0010<abstract><title style='display:none'>Abstract</title><p>Burnout syndrome has an increasing incidence among intensive therapists because of high expectations and stress which leads to physical, mental and emotional exhaustion. Our aim was to examine the causes and severity of burnout in intensive care units.</p><p>Maslach Burnout Inventory questionnaires were distributed among intensive care workers of an university and a town hospital. Socio-demographic data were also collected.</p><p>The questionnaires were completed by 67 professionals, 28 doctors, 39 nurses. 43.4% suffered serious burnout, 23.52% high level of depersonalization, only 19.11% were satisfied with their accomplishments. Females showed higher incidence of burnout, especially those with chronic diseases (OR=3.33). According to our data, burnout was not related to family status and working hours, however there was significant correlation between satisfaction and weekly relaxation time (p = 0.0115).</p><p>Burnout syndrome is spreading among intensive care employees, therefore its prevention should be a priority.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00International recognition of Ferenc Pápai Páriz as one of the first descriptors of all 4 cardinal signs of Parkinson’s diseasehttps://sciendo.com/article/10.2478/orvtudert-2018-0006<abstract><title style='display:none'>Abstract</title><p>Pax Corporis is the first book written in Hungarian that presents in detail human diseases, their etiology and treatment. Ferenc Pápai Páriz had completed the manuscript entitled Pax Corporis in 1687, and it was published 3 years later in 1690 in Kolozsvár. Ferenc Pápai Páriz summarized the knowledge he gained during his studies and accumulated during his personal practice. He did not write this book for the professionals but for those poor people who had no access to physician’s care. This was the reason why Pax Corporis was written in Hungarian. Whereas Pápai’s Latin language scripts – for example his doctoral thesis written in Basel – are known to the international scientific community, the Hungarian language Pax Corporis remained unknown for all who were not familiar with the language. For this reason it also remained neglected that in Pax Corporis Ferenc Pápai Páriz had given a detailed description of all four currently acknowledged cardinal signs of Parkinson’s disease – tremor, rigor, bradykinesia and postural instability – and also of other characteristics of the disease 130 years before James Parkinson. The report on the description of the syndrome of Parkinson’s disease in Pax Corporis was presented to the international professional community in 2009. In the current study we evaluated the international recognition of Ferenc Pápai Páriz as one of the first descriptors of all 4 cardinal signs of Parkinson’s disease. We searched scientific citation databases – Web of Science, Scopus, Google Scholar – and also performed general searches on the web. We found that until April 2018 Pápai Páriz has been cited among those who first described the complete motor syndrome of Parkinson’ disease in Pax Corporis, in many countries of the world from New Zealand to Canada, and also in 16 European countries. Citations appeared in dissertations, scientific publications, textbooks, handbooks, professional websites and other documents. Three centuries after his original Hungarian script, Ferenc Pápai Páriz got his international recognition in medical history as one of the first descriptors of the syndrome of Parkinson’s disease.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00The clinically important anaerobic, human pathogenic Bacteroides species and their antibiotic resistance levels in Central and Southeast Europehttps://sciendo.com/article/10.2478/orvtudert-2018-0003<abstract><title style='display:none'>Abstract</title><p>The <italic>Bacteroides</italic> and <italic>Parabacteroides</italic> species are important obligate anaerobic bacteria that are significant constituents of normal flora (microbiota), and opportunistic pathogens with special biological background. They are highly resistant to antibiotics and monitoring their resistance levels is important for their empiric therapy. Several antibiotic resistance studies were conducted in the USA and Europe and we have data for the region involved in this study showing comparable trends. Multidrug-resistant strains are emerging among Bacteroides too, where the proper antibiotic tests and treatments may be life-saving.</p></abstract>ARTICLE2019-01-25T00:00:00.000+00:00Occurrence of internal diseases in the premotor phase of Parkinson’s disease by analyzing a large database covering a whole populationhttps://sciendo.com/article/10.2478/orvtudert-2019-0009<p>The premotor phase of Parkinson’s disease (PD) precedes the appearance of motor symptoms by years. Many non-motor diseases have been associated with an increased risk of developing PD, but results of these studies are conflicting. The aim of this study was to investigate the occurrence of certain internal diseases (metabolic, circulatory, gastrointestinal) based on diagnosis codes, before the diagnosis of PD. There were 5209 patients included in the study who received diagnosis of PD at least in 2 years and we analyzed data retrospectively between 2004 and 2016. Out of metabolic diseases dyslipidemia (41%) and diabetes mellitus (32%), out of circulatory diseases hypertension (89%) and ischemic heart disease (51%) and out of gastrointestinal diseases gastroesophageal reflux disease (51%) and gallstones (25%) were the first two most common disorders in the examined PD patients. This is the first study in Hungary which analyzed PD in a large database in the context of internal diseases, and raised the possibility of a link between dyslipidemias, diabetes mellitus, hypertension, ischemic heart disease, gastooesophagial reflux, gallstones and PD.</p>ARTICLE2020-02-13T00:00:00.000+00:00en-us-1