rss_2.0The EuroBiotech Journal FeedSciendo RSS Feed for The EuroBiotech Journalhttps://sciendo.com/journal/EBTJhttps://www.sciendo.comThe EuroBiotech Journal 's Coverhttps://sciendo-parsed-data-feed.s3.eu-central-1.amazonaws.com/60cd715ef837f31a83f97d88/cover-image.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20210622T124853Z&X-Amz-SignedHeaders=host&X-Amz-Expires=604800&X-Amz-Credential=AKIA6AP2G7AKDOZOEZ7H%2F20210622%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Signature=efbab3a577c47ce9de8982036ba3da3d8fac36fdd5e95208b6db467f1c0154dd200300The situation of Covid-19 outbreak in Turkish Republic of Northern Cyprus, during the time of pandemic fatiguehttps://sciendo.com/article/10.2478/ebtj-2021-0014<abstract> <title style='display:none'>Abstract</title> <p>The coronavirus disease 2019 (COVID-19) outbreak has caused a massive impact on the entire world. Healthcare systems, economies, and social lives have already been overwhelmed. Turkish Republic of Northern Cyprus (TRNC), having been COVID-free for around two and a half months in the middle of the pandemic, is now facing a second wave of the infection after easing its preventive measures. Early implementation of precautions may dramatically reduce COVID-19 infections, but the virus is not eliminated. Additionally, the phenomenon "pandemic fatigue" introduced by the WHO is contributing to a significant decline in compliance with mitigation behaviors globally. With vaccines now rolling out, there is a reason to hope that there is an end in sight; however, it will most likely take a half year for widespread vaccinations to be in place. Decision-makers should analyze the effects of each intervention and pandemic fatigue to get ready for the further waves of infection.</p></abstract>ARTICLE2021-06-17T00:00:00.000+00:00Decision Analysis of the COVID-19 Vaccineshttps://sciendo.com/article/10.2478/ebtj-2021-0017<abstract> <title style='display:none'>Abstract</title> <p>The entire globe is struggling with the COVID-19 pandemic since March 11, 2020. There is still a large number of infected patients and death, and there is no proven treatment for the infection yet. This has led to the race in vaccine development to protect people from COVID-19 infection. As of February 3, 2021, there were 289 experimental COVID -19 vaccines in development, 66 of which were in clinical trials with different phases, and 20 of them were in phase 3.</p> <p>This study aims to evaluate 15 important vaccines based on criteria such as the dose number, dosing schedule, storage advantages, efficacy, and side effect. In this evaluation, we use the fuzzy PROMETHEE approach, which is an important Multi-Criteria Decision Making (MCDM) technique. The importance level of the criteria is determined based on expert opinion. The result shows that the EpiVacCorona vaccine is the most effective vaccine to prevent COVID-19 infections based on the selected criteria and the importance level of each criterion.</p> <p>The result obtained may change based on individual or expert’s priorities. Due to the use of different criteria for the ranking and different weightings of the criteria, the ranking result may differ. This study also shows the strengths and weaknesses of the selected vaccines and the applicability of the MCDM technique for the evaluation of COVID -19 vaccines.</p></abstract>ARTICLE2021-06-17T00:00:00.000+00:00 analysis of selected components of grapefruit seed extract against SARS-CoV-2 main proteasehttps://sciendo.com/article/10.2478/ebtj-2021-0015<abstract> <title style='display:none'>Abstract</title> <p>At the end of December 2019, first identified cases of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) started emerging. Ever since the emergence of the first case of infection with SARS-CoV-2 or COVID-19, it became the hottest research topic of numerous studies, in which scientists are trying to understand the path of infection, transmission, replication and viral action, all in order of finding a potential cure or vaccine applying various fundamental principles and methodologies. Using <italic>in silico</italic> method via AutoDock Vina 1.1.2., we analysed the binding affinity of six selected compounds from grapefruit seed extract (GSE) (narirutin, naringin, naringenin, limonin, ascorbic acid and citric acid) to SARS-CoV-2 main protease M<sup>pro</sup> (PDB ID: 6Y84), using acetoside, remdesivir and gallic acid as a positive controls of binding affinity. Results showed highest affinity (rmsd l.b. 0.000; rmsd u.b. 0.000) for narirutin (-10.5), then for naringin (-10.1), acetoside (-10.0), limonin (-9.9), remdesivir (-9.6), naringenin (-8.2), ascorbic acid (-6.7), citric acid (-6.4) and gallic acid (-6.4), all expressed in kcal/mol. Our findings suggest that selected compounds from grapefruit seed extract represent potential inhibitors of SARS-CoV-2 M<sup>pro</sup>, but further research is needed as well as preclinical and clinical trials for final confirmation of inhibitory functionality of these compounds.</p></abstract>ARTICLE2021-06-17T00:00:00.000+00:00IgA and IgG antibodies in SARS-CoV-2 vaccinated health workers by a homemade ELISA diagnostic testhttps://sciendo.com/article/10.2478/ebtj-2021-0016<abstract> <title style='display:none'>Abstract</title> <p>The SARS-CoV-2 pandemic has accelerated vaccine development and testing, an important step in its eradication. Health workers were included among the first subjects to be vaccinated, therefore it was necessary to check their immunological status after the first and second dose of vaccine.</p> <p>Serum samples belonging to 24 health workers undergoing vaccination for SARS-CoV-2 (Pfizer-BioNTech) were analysed: for 2 of them it was also possible to obtain a serum sample prior to the first dose of vaccine (zero time); antibody dosing was performed in 18 out of 24 after the first and second vaccination dose; in the remaining 6 healthcare workers a serum sample was obtained only after the second dose. In each serum sample, IgA and IgG antibodies to "Spike Receptor Binding Domain" (RBD) and "Nucleocapsid" (N) proteins were searched by ELISA test. Except for the two subjects for whom it was possible to have a serum sample before vaccination, the others were collected on day 18 from the first dose of vaccine and on day 8 from the second dose.</p> <p>Serum samples collected after the first dose of vaccine showed reactivity to anti-RBD IgG in 11 out of 18 healthcare workers and to IgA in 2 subjects. After the second dose all 24 samples showed the presence of anti-S IgG, while 5 revealed a reactivity for anti-S IgA. Three samples showed reactivity towards anti-N IgG.</p> <p>The ELISA test has shown all its effectiveness in controlling post vaccine immunity and in discriminating natural immunity from vaccine induced immunity.</p></abstract>ARTICLE2021-06-17T00:00:00.000+00:00Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and 60 subtyping of in diarrheic calves in Central Anatolia Region of Turkeyhttps://sciendo.com/article/10.2478/ebtj-2021-0010<abstract> <title style='display:none'>Abstract</title> <p>The aim of this study was to compare three diagnostic methods for the diagnosis of cryptosporidiosis and to detect subtypes of<italic>Cryptosporidium parvum</italic> by sequences analyses of <italic>gp60</italic> gene in diarrheic calves in several herds in Konya province located in Central Anatolia Region of Turkey. Fecal samples were collected from a total of 194 pre-weaned calves (n=158, ≤15 days old, and n=36, 15 to 40 days old), with diarrhoea. For comparative diagnosis, all samples were examined by modified Ziehl-Neelsen staining of fecal smears for the presence of oocyst, nested PCR-RFLP of SSU rRNA and TaqMan qPCR for the detection of <italic>Cryptosporidium</italic> DNA. A total of 92 (47.4%) and 104 (53.6%) out of the examined samples were found positive by microscopic examination and molecular tools, respectively. The diagnostic sensitivity and specificity of microscopic identification were determined as 88.5% and 100.0%, respectively compared to molecular assays. <italic>Cryptosporidium parvum</italic> was the only detected species in all positive samples by species-specific qPCR and nested PCR-RFLP assays. Species identifications were further confirmed by sequence analyses of the SSU rRNA PCR products. There was no statistically significant difference in <italic>C. parvum</italic> prevalence between early pre-weaned calves and calves older than 15 days. The sequence analyses of the <italic>gp60</italic> gene of <italic>C. parvum</italic> isolates revealed a one subtype IIaA13G2R1 belonging to zoonotic family IIa in diarrheic calves</p></abstract>ARTICLE2021-04-22T00:00:00.000+00:00The Association Between Serum Carnitine Level, Glucose Regulation, Body Fat and Nutrient Intake in Diabetic Individualshttps://sciendo.com/article/10.2478/ebtj-2021-0013<abstract> <title style='display:none'>Abstract</title> <p>Carnitine (β-hydroxy-γ-trimethyl amino butyrate) is, a vitamin-like substance carrying long-chain fatty acids into the mitochondrial matrix. Due to its effect in energy metabolism, carnitine plays an important role in controlling diabetes and its complications. Studies on this topic have often focused on carnitine supplementation. This study was planned to investigate the relationship between serum carnitine level, glucose regulation and body fat in diabetic patients. A total of 64 people between the ages of 30-5, 32 patients with type 2 diabetes and 32 healthy subjects, were included in the study. Individual lipid profiles, glucose, insulin and serum carnitine levels were analyzed, anthropometric measurements were taken and 24-hour recall food consumption was recorded. As a result, blood glucose, insulin, triglyceride, VLDL-C, HDL-C and HOMA-IR were found to be higher in diabetic individuals than healthy group (p&lt;0,05). Serum carnitine levels were found to be significantly lower in diabetic male (50,6±20,83 nmol/mL) than in healthy male (59,5±17,25 nmol/mL)(p&lt;0,05). This difference was not statistically significant among female (p&gt;0,05). It has been observed that intake of energy and macronutrients of diabetic individuals is generally lower than that of healthy individuals. Serum carnitine level was positively associated with polyunsaturated fatty acids and omega-6 fatty acid intake in male in the healthy group showed a negative correlation with fiber intake in female in the healthy group (p&lt;0,05). There were negative correlations between serum carnitine level with body weight, body mass index and body fat mass in female in the healthy group (p&lt;0,05). Individuals with diabetes are predisposed to dyslipidemia and insulin resistance. As a result; food consumption, and body fat affect individuals’ serum carnitine levels in type-2 diabetes. Since there is not enough study evaluating the relationship between anthropometric measurements of individuals and serum carnitine levels, it is thought that this result will guide future studies.</p> </abstract>ARTICLE2021-04-22T00:00:00.000+00:00Citric Acid Production of Yeasts: An Overviewhttps://sciendo.com/article/10.2478/ebtj-2021-0012<abstract> <title style='display:none'>Abstract</title> <sec id="j_ebtj-2021-0012_s_007"> <title style='display:none'>Background</title> <p>Citric acid, an intermediate product of the Krebs cycle, has a wide usage area in the food industry since it has some functions such as acidulant, flavouring agent, preservative and antioxidant. Although molds are the most commonly used microorganisms in the citric acid production, it is known that there are significant advantages of using yeasts.</p> </sec> <sec id="j_ebtj-2021-0012_s_008"> <title style='display:none'>Purpose and scope</title> <p>The microbial citric acid production mechanism needs to be well understood to make production more efficient. In this study, the yeasts used in the production, fermentation types and the factors affecting production were reviewed with studies.</p> </sec> <sec id="j_ebtj-2021-0012_s_009"> <title style='display:none'>Methodology</title> <p>Although production of citric acid can be produced by chemical synthesis, the fermentation is preferred because of its low cost and ease of use. More than 90% of citric acid produced in the world is obtained by fermentation.</p> </sec> <sec id="j_ebtj-2021-0012_s_010"> <title style='display:none'>Results</title> <p><italic>Yarrowia lipolytica</italic>, <italic>Candida zeylanoides</italic> and <italic>Candida oleophila</italic> are evaluated for citric acid production with substrates such as molasses, glucose, sucrose and glycerol. On the other hand, there is great interest in developing processes with new substrates and/ or microorganisms.</p> </sec> <sec id="j_ebtj-2021-0012_s_011"> <title style='display:none'>Conclusion</title> <p>Although the microbial strain is an important factor, the factors such as carbon, phosphorus and nitrogen sources, aeration, the presence of trace elements and pH are also parameters affecting the production.</p> </sec> </abstract>ARTICLE2021-04-22T00:00:00.000+00:00 production of sesquiterpenes by electro-elicitation in Gaetner calli and cell suspension cultureshttps://sciendo.com/article/10.2478/ebtj-2021-0011<abstract> <title style='display:none'>Abstract</title> <p>Aloeswood or Agarwood has long been perfumery commodity traded between Mediterranean Region and Southeast Asia since ancient times. Oil or chips are obtained by destructive harvesting of several Thymeleaceous genera includes <italic>Aquilaria</italic> and <italic>Gonystylus</italic> and are expensive and highly demanded ingredients in the global market. The recent recovery of <italic>Gyrinops walla</italic> as a potential producer of market-quality agarwood in mature damaged woods and branches, the intense illicit felling and exportation <italic>G. walla</italic> leading to the verge of extinction from Sri Lankan flora. The sustainable utilization of <italic>G. walla</italic> undoubtedly enhances the foreign exchange of the country and the non-destructive utilization <italic>G. walla</italic> through biotechnology is the only option available for sustainable exploitation and conservation of the vulnerable <italic>G. walla</italic> species. An electro-elicitation apparatuses were designed and built to stimulate calli and cell suspensions. The elicited and calli and cell suspensions were harvested at different time periods to extract sesquiterpenes. Current intensities of 10 mA and 50 mA, and 0.1 mA were the optimum values for the induction of sesquiterpenes; γ-selinene, β-caryophyllene, α-cadinol and α-guaiene, production in both calli and cell suspension after 8 weeks and 6 hours, respectively. The findings of the study led to conclude the possibility of induction of production of sesquiterpenes through electro-elicitation of <italic>G. walla</italic> calli and cell suspension in an in vitro system for sustainable utilization and conservation endeavours.</p></abstract>ARTICLE2021-04-22T00:00:00.000+00:00Detection of Biological Activity of Methanol Extract and Its Antibacterial effect on https://sciendo.com/article/10.2478/ebtj-2021-0009<abstract> <title style='display:none'>Abstract</title> <p>Plants are known to possess enormous profound benefits, which if well incorporated in daily living have the potential to influence our health in ways unexpected. Significant progress has been made concerning the anti-oxidative and anti-cancerous effect of bioactive components in food products, worthy of note in the curcuminoids derived from turmeric (<italic>Curcuma longa</italic>) rhizomes. Therefore, to this end, this research aims to describe succinctly the phytocomponents, antioxidant activity and anti-microbial activity of turmeric methanol extract. The zones of inhibition were measured after plating on a Mueller Hinton agar plate and compared to the standards penicillin G and chloramphenicol. Chloramphenicol used as a positive control was found to be most effective on <italic>Staphylococcus aureus</italic> while <italic>Curcuma longa</italic> methanol extract and penicillin G were found to have the same microbial inhibition due to the negative control. Compared to the IC50 values of Ascorbic acid which was 5 mg/ ml, Curcuma longa methanol extract had a lower IC50 value is 3.2 mg/ml which according to previous literature is a good one given that lower IC50 values indicate great antioxidant activity. It was gathered by the observation that the qualitative extraction of <italic>Curcuma longa</italic> using methanol shows the presence of varying chemical components such as AR-tumerone, arachinsaeure, alpha-tumerone amongst others. Research data suggest that ar-turmerone has the potential to promote the positive and negative proliferation of tumor cell lines. As a result, <italic>Curcuma longa</italic> has high potential to be developed into an antibiotic against Methicillin-resistant <italic>Staphylococcus aureus</italic> and other clinically important bacterial strains in future.</p></abstract>ARTICLE2021-04-22T00:00:00.000+00:00Biological effects induced by Gadolinium nanoparticles on Lymphocyte A20 cell linehttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.09<abstract><title style='display:none'>Abstract</title><p> Gadolinium nanoparticles (GdNPs) are potential agents for MRI of lymph nodes. The aim of this study was to evaluate the in vitro effects of 1 μM, 2.5 μM and 5 μM of GdDOTA⊂CS-TPP/HA and GdDOTP⊂CS-TPP/HA NPs on A20 lymphocyte cells exposed for 6 and 24 hours. The total cellular biomass (SRB), lactate dehydrogenase activity (LDH) and oxidative stress parameters, such as reactive oxygen species generation (ROS), reduced glutathione (GSH), malondialdehyde (MDA) and advanced oxidation protein products (AOPP) were analyzed by spectrophotometric and fluorimetric methods. After cells exposure to 1 μM, 2.5 μM and 5 μM of GdDOTP⊂CS-TPP/HA NPs their viability decreased in a time- and dose-dependent manner, whereas for GdDOTA⊂CS-TPP/HA no significant changes were noticed. Both NPs formulations in doses of 1 μM, 2.5 μM, 5 μM did not affect the plasma membrane at each time point tested. The levels of ROS, MDA and AOPP increased proportionally with the concentration and exposure time. GSH concentration decreased significantly for all doses of both NPs tested. Taken together our data suggest that, GdDOTP⊂CS-TPP/HA and GdDOTA⊂CS-TPP/HA NPs induced oxidative stress in A20 lymphocyte cells which was counteracted by the cells antioxidant defense system to a certain extend.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Antibody engineering and phage display technologyhttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.18<abstract><title style='display:none'>Abstract</title><p> Here we provide our pipeline for antibody discovery process. Starting from de novo isolation from custom made phage display libraries to expression and downstream applications; it allows us to obtain recombinant antibodies with desired properties to vast set of antigens. We also developed the CYB5-fusion system for periplasmic expression of multimeric proteins with the possibility of process monitoring. The combination of redox dependent absorbance spectrum of red-colored cytochrome b5 with its high value molar extinction coefficient allows us to monitor antibody fusion proteins localization, redox state and quantify them in reliable way in turbid solutions. Moreover, it was revealed that due to outstanding stability and solubility, cytochrome b5 significantly enhances expression level of antibody fragments in Escherichia coli periplasm.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Starter cultures of lactic acid bacteria for special diet productshttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.15<abstract><title style='display:none'>Abstract</title><p> From fresh and fermented vegetables (cucumber, sauercraut, eggplant), cereals (wheat, rice), legumes (soy) 37 cultures of lactic acid bacteria were isolated. Based on biochemical characteristics, MALDI-TOF MS protein profiling and 16S rRNA gene sequencing they were identified as representatives of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Enterococcus genera. Six members of Lactobacillus genera and two members of Leuconostoc genera actively producing EPS were selected for further investigation as components of starters for production of special dietetic foodstuffs.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Antibiotic resistance in lactococci and enterococci: phenotypic and molecular-genetic aspectshttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.03<abstract><title style='display:none'>Abstract</title><p> Extensive use of antibiotics in medicine, veterinary practice and animal husbandry has promoted the development and dissemination of bacterial drug resistance. The number of resistant pathogens causing common infectious diseases increases rapidly and creates worldwide public health problem. Commensal bacteria, including lactic acid bacteria of genera Enterococcus and Lactococcus colonizing gastrointestinal and urogenital tracts of humans and animals may act as vehicles of antibiotic resistance genes similar to those found in pathogens. Lactococci and enterococci are widely used in manufacturing of fermented products and as probiotics, therefore monitoring and control of transmissible antibiotic resistance determinants in industrial strains of these microorganisms is necessary to approve their Qualified Presumption of Safety status. Understanding the nature and molecular mechanisms of antibiotic resistance in enterococci and lactococci is essential, as intrinsic resistant bacteria pose no threat to environment and human health in contrast to bacteria with resistance acquired through horizontal transfer of resistance genes. The review summarizes current knowledge concerning intrinsic and acquired antibiotic resistance in Lactococcus and Enterococcus genera, and discusses role of enterococci and lactococci in distribution of this feature.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Editorialhttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.01ARTICLE2017-01-27T00:00:00.000+00:00Computational fluid dynamic (CFD) modeling of simultaneous extraction and fermentation process in a single sugar beet cossettehttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.04<abstract><title style='display:none'>Abstract</title><p> For simulations of flow and microbial conversion reactions, related to modeling of simultaneous extraction and fermentation process in a single sugar beet cossette a software package OpenFOAM was used. The mass transfer of the components (sucrose, glucose, fructose and ethanol) in the studied system was controlled by the convection and diffusion processes. Microbial conversion rates and yield coefficients were experimentally determined and/or estimated by mathematical simulation. Dimensions of the model sugar beet cossette (SBC) were: average length of cosettes 40.10 mm, average thickness 3.32 mm and average width 3.5 mm, and represented in the model as a square-shape cross-section mathematical simulation. Dimensions of the model sugar beet cossette (SBC) were: average length of cosettes 40.10 mm, average thickness 3.32 mm and average width 3.5 mm, and represented in the model as a square-shape cross-section used to study the mass transfer and microbial conversion rates on the scale of single sugar beet cossette in the short time scales (up to 25 s). This model can be used for simulation of extractant flow around single sugar beet cossette as well as for description of simultaneous extraction and fermentation process in the studied system.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Evaluation of results in research made in order to obtain a phytotherapeutic product for the prophylaxis and fight against nosema in beeshttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.06<abstract><title style='display:none'>Abstract</title><p> In order to obtain a natural apiphytotherapeutic formula with anti-parasite effect, as an alternative in the control of Nosema spp. infection, an original formula based on hydro-alcoholic extracts from plants and propolis was tested. Experiments were made on naturally infected adult bees from private apiaries diagnosed with nosemosis (Nosema apis/Nosema ceranae). Initially hydro-alcoholic extracts from plants in Romania’s flora and respectively from propolis (Apis mellifera), with sensorial, physical- chemical and microbiologic characteristics, in conformity with requirements in the European Pharmacopeia VI<sup>th</sup> Edition and Romanian Pharmacopeia X<sup>th</sup> edition. Parasitosis was demonstrated through laboratory methods, respectively through direct microscopic examination, on bee samples collected from aviaries according to O.I.E. (World Organisation for Animal Health) methodology (2008). „In vitro“ lab testing of plant extracts and of work variants was done firstly on naturally infested polyfloral honey having an average concentration of 7 spores/ microscopic field of Nosema sp., with the purpose of selecting the most effective work variant which was afterwards tested „in vivo“ in private apiaries, on bee colonies. The results of the clinical study showed the absence of disease in 75% of the treated hives (33 hives), bees being declared clinically healthy. In 14 % of the hives (6 hives) infestation was weak, in 6.82% of the hives (3 hives) infestation was medium, and 4.55 % (2 hives) were diagnosed with a massive degree of infestation and the therapeutic protocol continued.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Prosopis juliflora (mesquite) gum exudate as a potential excipienthttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.12<abstract><title style='display:none'>Abstract</title><p> Gum exudate was obtained from Prosopis juliflora (Sw.) DC., which is abundantly available in north-west, central, west and south India. It was analysed for its phytochemical composition in aqueous extract and as well as by LCMS, GCMS, TGDTA and SEM to validate it’s potential for use as an excipient (Fig. 1).</p><p><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/7600_ebtj_76.jpg"/></p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Methodological approach to the study of dynamics of specific concentration of cell wall antigens per cell of Bacillus species and examples of its applicationhttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.10<abstract><title style='display:none'>Abstract</title><p> Background: Nonpathogenic Bacillus strains are used in biotechnology, and pathogenic Bacillus strains are cause of food borne disease. It explains the relevance of the methods of detection and quantification of whole cell and cell components of these bacteria. Aims: Development of methodological approach for investigation of dynamics of specific concentration of cell wall antigens per cell of bacilli without solubilization of cell wall during sample preparation; using of the approach with 6 strains of bacilli as an example. Method: ELISA. Results: Methodological approach for investigation of dynamics of specific concentration of bacilli cell wall antigens has been developed. The distinctive features of the approach are rabbit polyclonal antibodies to genera-specific antigens of bacilli as key reagent and lack of need for solubilization of cell wall during sample preparation. It was shown using 6 strains of Bacilli as an example that specific concentration of cell wall antigens per cell vary according to bacillus strain, stage of culture growth and media composition. The data will find an application in biotechnology of clinical diagnostics and test-systems for food control including detection of whole bacillus cells.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Applying gas chromatography to monitor extracellular free amino acids content in cultivation medium during lactic acid fermentationhttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.05<abstract><title style='display:none'>Abstract</title><p> The aim of this work was the adaptation of a Gas Chromatographic-Flame Ionization Detector (GC-FID) method for detection and quantification of extracellular free amino acids in demineralized water, De Mann Rogosa Sharpe (MRS) medium and corn grits (CG) withdrawn during lactic acid fermentation. In order to analyze free amino acids by the GC-FID method it was necessary to convert free amino acids to volatile compounds. This was accomplished by derivatization of free amino acids with ethylchlor formate in aqueous medium followed by extraction of volatile free amino acid esters with chloroform. It was proven that the combination of derivatization and extraction procedure with developed GC-FID method gave accurate, reproducible and sensitive analytical results. Quantification of 15 (Ala, Gly, Val, Leu, Ile, Phe, Tyr, Trp, Asn, Met, Pro, Lys, His, Asp and Glu) out of 20 ethoxycarbonyl-ethyl esters of free amino acids in demineralized water and MRS medium was achieved by established methods. In corn grits medium all of the above mentioned 15 amino acids, except His, were quantified with this GC-FID method. The established method was efficiently verified in monitoring of extracellular free amino acid concentration during lactic acid production with Lactobacillus rhamnosus DSM 20021<sup>T</sup> in MRS medium and Lactobacillus amylovorus DSM 20531<sup>T</sup> in corn grits medium.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00Studies and research regarding acclimatization and breeding of new vegetable plant, Momordica charantia at V.R.D.S. Buzău, Româniahttps://sciendo.com/article/10.24190/ISSN2564-615X/2017/01.13<abstract><title style='display:none'>Abstract</title><p> Between 1996-2015, at V.R.D.S Buzau were conducted researches regarding the acclimatization, breeding and elaboration of crop technology for a new vegetable species, Momordica charantia (bitter cucumber). There were purchased and studied a large number of genotypes which were assessed for a long period; 4 of those have demonstrated increased adaptability, for which they were retained and multiplied in lineage. In time, by specific cucurbits improvement techniques were obtained 4 genetically stabilized accessions noted as G1, early accessions with large fruits,G2, medium fruit size, G3, medium fruit size and L4 with small crunchy fruits, semi tardy. Of these, L3 was homologated and patented under the name of Rodeo, and the other 3 will be proposed for homologation in the future. The studies demonstrated that this species has well adapted to our pedoclimatic conditions; it can be cultivated both in greenhouses and in open fields, in warmer areas, mentioning that for both crop variants it is recommended a drilling system. The new genotypes support various technological variants and can be ecologically cultivated with remarkable results.</p></abstract>ARTICLE2017-01-27T00:00:00.000+00:00en-us-1