rss_2.0Asian Biomedicine FeedSciendo RSS Feed for Asian Biomedicine Biomedicine 's Cover–Lo Presti criteria modified by body surface area for the electrocardiographic diagnosis of left ventricular hypertrophy in Thai patients<abstract> <title style='display:none'>Abstract</title> <sec><title style='display:none'>Background</title> <p>Several electrocardiographic (ECG) criteria are used to diagnose left ventricular hypertrophy (LVH); however, they have low sensitivity.</p> </sec> <sec><title style='display:none'>Objective</title> <p>To assess the sensitivity of LVH diagnosis using Peguero–Lo Presti criteria modified by body surface area (BSA).</p> </sec> <sec><title style='display:none'>Methods</title> <p>This study used retrospective data from 9,438 patients who attended the Central Chest Institute of Thailand from January 2017 to December 2017 with available echocardiography, and who were categorized into those with and without LVH to determine diagnostic accuracy. We randomly selected 317 patients after excluding others based on various conditions. The left ventricular mass of the 317 patients was estimated using echocardiography. Peguero–Lo Presti criteria were modified by dividing original criteria by BSA. The accuracy of the modified criteria was compared with that of the original Peguero–Lo Presti, Sokolow–Lyon, and Cornell voltage criteria. A McNemar test was used to determine the agreement of all ECG criteria examined with LV mass index. The area under a receiver operating characteristic curve (AUC) was used to assess the performance of the criteria.</p> </sec> <sec><title style='display:none'>Results</title> <p>LVH was diagnosed in 164 of the 317 patients using echocardiography. The sensitivity of modified Peguero–Lo Presti criteria was 50.6% (95% confidence interval [CI] 42.7% to 58.5%), and specificity was 88.2% (95% CI 82.0% to 92.9%), with an AUC of 0.67 (95% CI 0.61–0.73).</p> </sec> <sec><title style='display:none'>Conclusions</title> <p>Peguero–Lo Presti criteria modified by dividing them by BSA can improve sensitivity with acceptable specificity for the diagnosis of LVH compared with other ECG criteria examined, at least in selected Thai patients. The modified Peguero–Lo Presti criteria have accuracy similar to that for the original criteria.</p> </sec> </abstract>ARTICLE2021-04-30T00:00:00.000+00:00Cytogenetic characteristics of and prognosis for acute myeloid leukemia in 107 children<abstract> <title style='display:none'>Abstract</title> <sec><title style='display:none'>Background</title> <p>Patients diagnosed with acute myeloid leukemia (AML) in childhood have a poor prognosis. A better understanding of prognostic factors will assist patients and clinicians in making difficult treatment decisions.</p> </sec> <sec><title style='display:none'>Objectives</title> <p>To understand further the cytogenetic characteristics of and reassess the prognostic value of cytogenetic abnormalities in childhood AML.</p> </sec> <sec><title style='display:none'>Methods</title> <p>Conventional karyotypes of 107 children with AML were analyzed retrospectively. The cases were divided into 4 groups based on genetic rearrangements; namely patients with: t(15;17)/<italic>PML-RARA</italic>; t(8;21)/<italic>RUNX1-RUNX1T1</italic> or inv(16)(p13;q22) and t(16;16)/<italic>CBFB-MYH11</italic>; −7 or complex karyotypes; normal karyotypes or other cytogenetic changes. Differences in age, sex, leukocyte count, event-free survival (EFS), and overall survival (OS) were analyzed.</p> </sec> <sec><title style='display:none'>Results</title> <p>All French-American-British (FAB) subtypes of AML were detected in 107 patients. We successfully cultured 81 of 107 bone marrow specimens, of which 60 cases had abnormal karyotypes. The most common abnormal karyotypes were t(8;21) (17/81 cases), followed by t(15;17) (13/81 cases), –X/Y (10/81 cases). There were no significant differences (<italic>P</italic> &gt; 0.05) in age, sex, or leukocyte counts between the 4 groups. The differences in 3-year EFS and OS between each pair were significant, except for groups of patients with t(8;21)/<italic>RUNX1-RUNX1T1</italic> and patients with normal karyotypes or other cytogenetic changes (<italic>P</italic> = 0.054).</p> </sec> <sec><title style='display:none'>Conclusions</title> <p>Chromosomal abnormalities may provide important prognostic factors for AML in children and be helpful for risk stratification and individual treatment.</p> </sec> </abstract>ARTICLE2021-04-30T00:00:00.000+00:00Primary culture of chondrocytes after collagenase IA or II treatment of articular cartilage from elderly patients undergoing arthroplasty<abstract> <title style='display:none'>Abstract</title> <sec><title style='display:none'>Background</title> <p>Joint replacement surgery provides articular cartilage samples for chondrocyte isolation. To our knowledge, the effect of the collagenase type on releasing of chondrocytes from the extracellular matrix of cartilage is not reported.</p> </sec> <sec><title style='display:none'>Objectives</title> <p>To determine whether cartilage digested with collagenase IA yielded more chondrocytes than that digested with collagenase II and determine whether chondrocytes isolated with collagenase IA could be cultured in vitro.</p> </sec> <sec><title style='display:none'>Methods</title> <p>Cartilage slices collected from 18 elderly patients who received joint replacement surgery (16 hips, 2 knees) were digested sequentially with 0.4% pronase E and 0.02% collagenase IA, or with 0.15% collagenase II alone, or sequentially with 0.4% pronase E and 0.02% collagenase II. We compared cell yield from each method. Cell viability by the most effective method was calculated and plotted. The morphology of cultured monolayer chondrocytes was recorded with a light microscope.</p> </sec> <sec><title style='display:none'>Results</title> <p>Sequential digestion with pronase E and collagenase IA yielded 2566 ± 873 chondrocytes per mg wet cartilage, which was more effective than the other isolation methods (<italic>P</italic> = 0.018). The average chondrocyte viability could reach 84% ± 8% (n = 11). Light microscopic images showed typical chondrocyte morphology in monolayer cultures.</p> </sec> <sec><title style='display:none'>Conclusion</title> <p>Sequential digestion of human articular cartilage with pronase E and collagenase IA was more effective than collagenase II alone or collagenase II combined with pronase E for releasing chondrocytes from extracellular matrix of cartilage. Chondrocytes isolated with this method could be maintained in monolayer cultures for at least 2 passages with unaltered morphology.</p> </sec> </abstract>ARTICLE2021-04-30T00:00:00.000+00:00Association of industrial work schedules with development of metabolic syndrome, insulin resistance, and serum adipokine concentrations<abstract> <title style='display:none'>Abstract</title> <sec><title style='display:none'>Background</title> <p>Association of work schedule in industrial workers with the progression of metabolic syndrome, insulin resistance, and serum adipokine concentrations is incompletely explored.</p> </sec> <sec><title style='display:none'>Objective</title> <p>To determine the association of work schedule with the progression of metabolic syndrome, insulin resistance, and adipokine concentrations in industrial workers.</p> </sec> <sec><title style='display:none'>Methods</title> <p>In a cross-sectional study design of industrial workers we compared metabolic syndrome, insulin resistance, and adipokines concentration between workers in the day shift (n = 52), rotational shift (n = 21), and night shift (n = 15). The international Diabetes Federation criteria were used to diagnose metabolic syndrome. We used a homeostatic model assessment of insulin resistance (HOMA-IR). Serum insulin, leptin, and adiponectin concentrations were measured using enzyme-linked immunosorbent assays. Serum glucose, triglyceride, and high-density lipoprotein cholesterol (HDL-C) concentrations were monitored using Prietest clinical chemistry reagents.</p> </sec> <sec><title style='display:none'>Results</title> <p>The proportional difference in metabolic syndrome (0.31, 95% confidence interval [CI] 0.036–0.587, <italic>P</italic> = 0.026), median difference of leptin (0.61, 95% CI 0.186–1.034, <italic>P</italic> = 0.005), and leptin-to-adiponectin ratio (LAR; 0.45, 95% CI 0.235–0.665, <italic>P</italic> &lt; 0.001) was significantly higher, and serum adiponectin was lower (–2.00, 95% CI −4.197 to 0.197, <italic>P</italic> = 0.07) in the night-shift workers compared with that of day-shift workers. Among rotational-shift workers, the proportional difference between metabolic syndrome (0.14, 95% CI −0.098 to 0.378, <italic>P</italic> = 0.25), median difference of leptin (0.25, 95% CI −0.124 to 0.624, <italic>P</italic> = 0.19), and LAR (0.09, 95% CI −0.099 to 0.279, <italic>P</italic> = 0.35) was higher, and serum adiponectin concentration was lower (−0.73, 95% CI −2.660 to 1.208, <italic>P</italic> = 0.46) compared with that of day-shift workers; however, the altered differences were not significant. We observed a higher proportion of difference in HOMA-IR in shift workers (night and rotation) than in day-shift workers.</p> </sec> <sec><title style='display:none'>Conclusion</title> <p>Night-shift workers are vulnerable to a higher risk of metabolic syndrome, HOMA-IR, and adipokine changes.</p> </sec> </abstract>ARTICLE2021-04-30T00:00:00.000+00:00COVID-19 immunity and vaccines: what a pharmacist needs to know<abstract> <title style='display:none'>Abstract</title> <p>COVID-19 vaccines are being produced using different platforms by different companies, some of which are entering Phase 3 and 4 trials. Due to the pandemic, this production has been accelerated, which leaves a window for speculation on the method of production and safety. Pharmacists are familiar with vaccination; however, COVID-19 vaccines are still new and further work is needed to clarify many aspects, including side effects, methods of storage, and number of doses. Prioritization of vaccination has been implemented to a certain extent, but no clear strategy is available. A comprehensive overview on immunity and immunological principles for the design of COVID-19 vaccine strategies is provided in this narrative review and the current COVID-19 vaccine landscape is discussed, in addition to exploring the principles for prioritization of vaccination using data from articles available in PubMed and from health organizations. Pharmacists should have a better understanding of COVID-19 vaccines and their manufacture. This would also allow better counseling of the public on COVID 19, immunization, and explaining prioritization basis and vaccination programs.</p> </abstract>ARTICLE2021-04-30T00:00:00.000+00:00Health risks among shift workers: the need for a multipronged approach of herbal medicines among the elderly in a primary care unit in Hat Yai, Songkhla province, Thailand<abstract> <title style='display:none'>Abstract</title> <sec><title style='display:none'>Background</title> <p>The prevalence of chronic diseases in the elderly is high, and some use herbal medicines instead of, or together with, conventional medicine. Herbal medicine usage may cause adverse events.</p> </sec> <sec><title style='display:none'>Objectives</title> <p>To determine the prevalence of herbal medicine usage among the elderly attending a primary care unit (PCU) of Songklanagarind Hospital, a tertiary teaching hospital in Hat Yai, Songkhla province, Thailand, and associated factors, reasons for use, principles considered before use, perceived effects, and history of consultation with medical professionals concerning their herbal medicine usage.</p> </sec> <sec><title style='display:none'>Methods</title> <p>We conducted a cross-sectional survey of a convenience sample of elderly patients who visited the PCU. Questionnaires were used to obtain data.</p> </sec> <sec><title style='display:none'>Results</title> <p>We included 204 patients who met the eligibility criteria. About two-thirds were women, with a median age of 69.0 years. Most had underlying diseases and were educated. The all time prevalence of herbal medicine usage was 60.8%. Being educated was significantly associated with herbal medicine usage. Among 124 users, 79% did not consider any principles for safe use of herbal medicine, 63% had no knowledge of possible interactions with conventional medicines, and 73% had never been asked about their use of herbal medicines by their health care professionals.</p> </sec> <sec><title style='display:none'>Conclusions</title> <p>There is a high prevalence of herbal medicine usage among the elderly Thai patients attending the PCU, especially by the educated. The majority did not consider the principles for safe use of herbal medicine. Health care providers should be more aware of herbal medicine usage and should increase their role in initiating a discussion about herbal medicine usage with elderly patients.</p> </sec> </abstract>ARTICLE2021-02-21T00:00:00.000+00:00Stem cell transplantation therapy for diabetic foot ulcer: a narrative review<abstract> <title style='display:none'>Abstract</title> <p>Diabetes mellitus is a chronic metabolic disease associated with high cardiovascular risk. A vascular complication of diabetes is foot ulcers. Diabetic foot ulcers are prevalent and substantially reduce the quality of life of patients who have them. Currently, diabetic foot ulcer is a major problem for wound care specialists, and its treatment requires considerable health care resources. So far, various therapeutic modalities have been proposed to treat diabetic foot ulcers and one of them is stem cell-based therapy. Stem cell-based therapy has shown great promise for the treatment of diabetic foot ulcers. This strategy has been shown to be safe and effective in both preclinical and clinical trials. In this review, we provide an overview of the stem cell types and possible beneficial effects of stem cell transplantation therapy for diabetic foot ulcers, and an overview of the current status of stem cell research in both preclinical and clinical trial stages of treatment strategies for diabetic foot ulcers.</p> </abstract>ARTICLE2021-02-21T00:00:00.000+00:00Atypical complication in an adult patient with dengue and autoimmune hemolytic anemia: a case report<abstract> <title style='display:none'>Abstract</title> <p>Severe dengue infection is associated with life-threatening complications, including severe bleeding. The bleeding tendency is typically associated with the shock phase of infection, for which blood replacement may be needed. However, repetitive blood transfusion can lead to volume overload. Administration of recombinant activated factor VII (rFVIIa) might be used to counteract bleeding without inducing volume overload. We describe the case of a patient with severe dengue infection who presented with intractable bleeding; he was initially treated with massive blood transfusions, which resulted in volume overload. He was then treated with rFVIIa to reverse the bleeding. During the second week of his hospitalization, his hematocrit dropped precipitously, and autoimmune hemolytic anemia was diagnosed. Supportive treatment was provided until recovery. Autoimmune hemolytic anemia is a rare complication in adult patients with dengue. Supportive care was effective for this atypical complication.</p> </abstract>ARTICLE2021-02-21T00:00:00.000+00:00Sensitivity and specificity of using pelvic ultrasonographic parameters combined with basal gonadotropin levels to diagnose central precocious puberty in Thai girls<abstract> <title style='display:none'>Abstract</title> <sec><title style='display:none'>Background</title> <p>The criterion standard gonadotropin-releasing hormone (GnRH) stimulation tests to diagnose central precocious puberty (CPP) are time-consuming, inconvenient, and expensive.</p> </sec> <sec><title style='display:none'>Objectives</title> <p>To determine predictive cut-off values codetermined by ultrasonographic parameters and basal gonadotropin levels in girls with premature sexual development and compare them results of criterion standard tests in a study of diagnostic accuracy.</p> </sec> <sec><title style='display:none'>Methods</title> <p>Retrospective review of hormonal investigations and ultrasonographic uterine and ovarian parameters in a consecutive sample of girls at a single center, tertiary care hospital in Bangkok, Thailand.</p> </sec> <sec><title style='display:none'>Results</title> <p>We separated data from 68 girls (age range 2–12 years) into 2 groups based on their response to a GnRH analogue agonist stimulation test. A “prepubertal response” group included girls with premature thelarche and thelarche variants (n = 18, 6.37 ± 1.77 years) and a “pubertal response” group, including girls with CPP (n = 50, 8.46 ± 1.46 years); excluding patients with pathological causes (n = 0). The basal level of luteinizing hormone (LH) had the largest area under receiver operating characteristic curves (AUC) of 0.84; 95% confidence interval [CI] 0.74–0.93) compared with basal levels of follicle stimulating hormone (AUC 0.77; 95% CI 0.64–0.90) or estradiol (0.70; 95% CI 0.56–0.85). An optimal cut-off of 0.25 IU/L LH was related to a pubertal response to GnRH analogue agonist stimulation tests with 75.0% sensitivity, 88.9% specificity, 94.7% positive predictive value (PPV), and 57.1% negative predictive value. Uterine and ovarian cut-off volumes of 3.5 cm<sup>3</sup> and 1.5 cm<sup>3</sup> were related to a pubertal response with 88.6% and 76.2% PPV, respectively. A uterine width cut-off of 1.7 cm combined with a basal LH cut-off of 0.25 IU/L increased specificity and PPV to 100%.</p> </sec> <sec><title style='display:none'>Conclusion</title> <p>Combining uterine and ovarian ultrasonographic parameters with basal gonadotropin levels, especially uterine width and basal LH level, appears useful for diagnosis of CPP.</p> </sec> </abstract>ARTICLE2021-02-21T00:00:00.000+00:00Diabetic foot ulcers: prevention is better than treatment calcium phosphate and styrene–butadiene polymer-based root canal filling material<abstract> <title style='display:none'>Abstract</title> <sec><title style='display:none'>Background</title> <p>Three-dimensional obturation of the root canal system is mandatory for a successful root canal treatment. Using a filling material with optimal properties may enable the root canal to be sealed well and therefore obtain the desired obturation.</p> </sec> <sec><title style='display:none'>Objective</title> <p>To develop a new injectable paste endodontic filling material using calcium phosphate powder and a styrene–butadiene emulsion polymer.</p> </sec> <sec><title style='display:none'>Methods</title> <p>The powder phase comprised an equivalent molar ratio of tetracalcium phosphate, anhydrous dicalcium phosphate, bismuth oxide, and calcium chloride. The liquid phase comprised a styrene–butadiene rubber emulsion in distilled water. The powder and the liquid were mixed to achieve a paste consistency. The paste was subjected to various tests including flow, setting time, dimensional change, solubility, and radiopacity to indicate its suitability as a root canal filling material. All these tests were conducted according to the American National Standards Institute–American Dental Association for endodontic sealing materials. After passing these tests, the paste was submitted to an injectability test.</p> </sec> <sec><title style='display:none'>Results</title> <p>The material showed acceptable flowability with 19.1 ± 1.3 min setting time and 0.61 ± 0.16% shrinkage after 30 days of storage. We found the highest solubility at 24 h (6.62 ± 0.58%), then the solubility decreased to 1.09 ± 0.08% within 3 days. The material was more radiopaque than a 3 mm step on an aluminum wedge. Furthermore, the material showed good injectability of 93.67 ± 1.80%.</p> </sec> <sec><title style='display:none'>Conclusions</title> <p>The calcium phosphate powder in styrene–butadiene emulsion met basic requirements for a root canal filling material with promising properties.</p> </sec> </abstract>ARTICLE2021-02-21T00:00:00.000+00:00Prevention of mother-to-child transmission of human immunodeficiency virus—tremendous progress despite remaining challenges Thai sisters with growth hormone insensitivity or Laron syndrome<abstract><title style='display:none'>Abstract</title><sec id="j_1905-7415.1102.549_s_005_w2aab3b7b3b1b6b1aab1c15b1Aa"><title style='display:none'>Background</title><p>Growth hormone insensitivity (GHI) or Laron syndrome can result from GH receptor (GHR) or postreceptor defects, such as in GH binding or transduction, or insulin-like growth factor 1 (IGF-1) synthesis. Multiple defects in GHI have been reported in cohorts from the Middle East, Ecuador, and the Mediterranean, but rarely reported from Southeast Asia.</p></sec><sec id="j_1905-7415.1102.549_s_006_w2aab3b7b3b1b6b1aab1c15b2Aa"><title style='display:none'>Methods</title><p>Genomic DNA was isolated from peripheral blood leukocytes of young Thai sisters with severe short stature. Coding exons, including the intronic boundaries of the GHR were amplified from genomic DNA by PCR, and products were purified and sequenced. Serum GH, IGF-1, and IGF binding protein-3 were assayed immunometrically.</p></sec><sec id="j_1905-7415.1102.549_s_007_w2aab3b7b3b1b6b1aab1c15b3Aa"><title style='display:none'>Results</title><p>We found an extreme GHI phenotype and a homozygous mutation in exon 7 of GHR.</p></sec><sec id="j_1905-7415.1102.549_s_008_w2aab3b7b3b1b6b1aab1c15b4Aa"><title style='display:none'>Conclusions</title><p>This mutation can cause a new donor splice site and interfere with mRNA splicing. To our knowledge, these are first cases of Laron syndrome in Thais confirmed by genotyping.</p></sec></abstract>ARTICLE2017-08-31T00:00:00.000+00:00Thai national guidelines for the prevention of mother-to-child transmission of human immunodeficiency virus 2017<abstract><title style='display:none'>Abstract</title><sec id="j_1905-7415.1102.547_s_020_w2aab3b7c23b1b6b1aab1c15b1Aa"><title style='display:none'>Background</title><p>Thailand has made progress in reducing perinatal HIV transmission rates to levels that meet the World Health Organization targets for so-called “elimination” (&lt;2%) of mother-to-child transmission (MTCT).</p></sec><sec id="j_1905-7415.1102.547_s_021_w2aab3b7c23b1b6b1aab1c15b2Aa"><title style='display:none'>Objectives</title><p>To highlight the Thailand National Guidelines on HIV/AIDS Treatment Prevention Working Group issued a new version of its National Prevention of MTCT guidelines in March 2017 aimed to reduce MTCT rate to &lt;1% by 2020.</p></sec><sec id="j_1905-7415.1102.547_s_022_w2aab3b7c23b1b6b1aab1c15b3Aa"><title style='display:none'>Discussion of guidelines</title><p>The guidelines include recommending initiation of antepartum antiretroviral therapy (ART) containing tenofovir disoproxil fumarate (TDF) plus lamivudine (3TC)/emtricitabine (FTC) plus efavirenz regardless of CD4 cell count as soon as HIV is diagnosed for ART naïve HIV-infected pregnant women. An alternative regimen is TDF or zidovudine (AZT) plus 3TC/FTC plus lopinavir/ritonavir (LPV/r) for HIV-infected pregnant women suspected resistant to non-nucleoside reverse transcriptase inhibitors. Treatment should be started immediately irrespective of gestational age and continued after delivery for life. Raltegravir is recommended in addition to the ART regimen for HIV-infected pregnant women who present late (gestational age (GA) ≥32 weeks) or those who have a viral load (VL) &gt;1000 copies/mL at GA ≥32 weeks. HIV-infected pregnant women who conceive while receiving ART should continue their treatment regimen during pregnancy. HIV-infected pregnant women who present in labor and are not receiving ART should receive single-dose nevirapine immediately along with oral AZT, and continue ART for life. Infants born to HIV-infected mothers are categorized as high or standard risk for MTCT. High MTCT risk is defined as an infant whose mother has a viral load (VL) &gt; 50 copies/mL at GA &gt; 36 weeks or has received ART &lt;12 weeks before delivery, or has poor ART adherence. These infants should be started on AZT plus 3TC plus NVP for 6 weeks after delivery. Infants with standard MTCT risk should receive AZT for 4 weeks. Formula feeding exclusively is recommended for all HIV-exposed infants.</p></sec></abstract>ARTICLE2017-08-31T00:00:00.000+00:00Congenital abdominal aortic aneurysm in a term neonate: a case report<abstract><title style='display:none'>Abstract</title><sec id="j_1905-7415.1102.548_s_001_w2aab3b7b6b1b6b1aab1c14b1Aa"><title style='display:none'>Background</title><p>Congenital abdominal aortic aneurysm (AAA) is a rare condition in neonates. To our knowledge, the natural course of the disease in a Thai neonate has not yet been reported.</p></sec><sec id="j_1905-7415.1102.548_s_002_w2aab3b7b6b1b6b1aab1c14b2Aa"><title style='display:none'>Objectives</title><p>To report the characteristics and clinical course of congenital AAA in a Thai neonate.</p></sec><sec id="j_1905-7415.1102.548_s_003_w2aab3b7b6b1b6b1aab1c14b3Aa"><title style='display:none'>Methods</title><p>A female Thai infant was born spontaneously at term (3,990 g) having a large, pulsatile, abdominal mass. Computed tomographic angiography (CTA) of the abdominal aorta showed a large infrarenal AAA, and a fusiform aneurysm at the left common iliac artery. Two small right renal arterial aneurysms were also noted. The large aneurysm was partially resected and a Gore-Tex vascular graft was placed at 15 days old. Histopathology of the aneurysmal wall revealed no specific etiology. Ultrasonography revealed thrombosis of the graft on the 13th day after surgery.</p></sec><sec id="j_1905-7415.1102.548_s_004_w2aab3b7b6b1b6b1aab1c14b4Aa"><title style='display:none'>Results</title><p>Repeated CTA of the abdominal aorta at age 13 mo showed complete thrombosis of the graft with reconstitution of collateral circulation. The infrarenal AAA and left common iliac aneurysm and 2 small right renal artery aneurysms were completely thrombosed. The patient grew and developed normally to the most recent follow up at age 36 mo.</p></sec><sec id="j_1905-7415.1102.548_s_005_w2aab3b7b6b1b6b1aab1c14b5Aa"><title style='display:none'>Conclusions</title><p>Congenital AAA with failure of graft replacement may regress spontaneously.</p></sec></abstract>ARTICLE2017-08-31T00:00:00.000+00:00Anti-inflammatory effect of the taffy , made from the Korean radish L. in a lipopolysaccharide-induced murine model of pulmonary inflammation<abstract><title style='display:none'>Abstract</title><sec id="j_1905-7415.1102.546_s_001_w2aab3b7c12b1b6b1aab1c15b1Aa"><title style='display:none'>Background</title><p>Korean radish (<italic>Raphanus sativus</italic> L.) is used in <italic>kimchi</italic>, a staple of Korean cuisine. A taffy, <italic>mu yeot</italic>, that includes mainly radish together with other herbs (Bulbus Allii Sativi, Fructus Zizyphi, Semen Zizyphi, Rhizoma Zingberis, and Radix Glycyrrihizae) is used traditionally in Korea to improve pulmonary symptoms. However, to our knowledge, these effects of the taffy have not yet been studied systematically.</p></sec><sec id="j_1905-7415.1102.546_s_002_w2aab3b7c12b1b6b1aab1c15b2Aa"><title style='display:none'>Objectives</title><p>To elucidate the anti-inflammatory effects of radish taffy on macrophages and a murine model of pulmonary inflammation, and to suggest the most likely candidate for the effects.</p></sec><sec id="j_1905-7415.1102.546_s_003_w2aab3b7c12b1b6b1aab1c15b3Aa"><title style='display:none'>Methods</title><p>A macrophage cell line, RAW264.7, was used to examine effects of the radish taffy in vitro. Pulmonary inflammation was assessed 24 h after oral instillation of lipopolysaccharide in mice treated with radish taffy for 10 days. We determined the chemical components of radish taffy quantitatively using tandem HPLC mass spectroscopy.</p></sec><sec id="j_1905-7415.1102.546_s_004_w2aab3b7c12b1b6b1aab1c15b4Aa"><title style='display:none'>Results</title><p>Radish taffy had no apparent effect on the RAW264.7 cells. Treatment of the mice with radish taffy significantly reduced the recruitment of neutrophils to the lung, and the level of myeloperoxidase in bronchoalveolar lavage fluid (BALF). ELISAs showed that the treatment significantly decreased the level of IL 6 in BALF induced by LPS, but not the levels of IFNγ, TNFα, IL 10, or IL 12. Nor did the taffy change the levels of those cytokines or NF-κB activation in lung homogenates. HPLC-MS suggested glycyrrhizin as the most likely candidate for the anti-inflammatory effects.</p></sec><sec id="j_1905-7415.1102.546_s_005_w2aab3b7c12b1b6b1aab1c15b5Aa"><title style='display:none'>Conclusions</title><p>Radish taffy suppresses neutrophil recruitment to lungs of mice, possibly by reducing IL 6 levels, which may lead to reduced pulmonary inflammation.</p></sec></abstract>ARTICLE2017-08-31T00:00:00.000+00:00Clipless laparoscopic adrenalectomy for pheochromocytoma<abstract><title style='display:none'>Abstract</title><sec id="j_1905-7415.1102.545_s_001_w2aab3b7b1b1b6b1aab1c15b1Aa"><title style='display:none'>Background</title><p>Laparoscopic adrenalectomy remains a challenging operation for pheochromocytoma (PCC) because of excessive secretion of catecholamines causing hemodynamic instability.</p></sec><sec id="j_1905-7415.1102.545_s_002_w2aab3b7b1b1b6b1aab1c15b2Aa"><title style='display:none'>Objectives</title><p>To evaluate the use of the LigaSure vessel sealing system (Covidien-Medtronic) to secure hemostasis during laparoscopic adrenalectomy for PCC.</p></sec><sec id="j_1905-7415.1102.545_s_003_w2aab3b7b1b1b6b1aab1c15b3Aa"><title style='display:none'>Methods</title><p>In this observational study we retrospectively reviewed a case series of 19 patients with preoperatively diagnosed PCC, who underwent laparoscopic adrenalectomy who underwent laparoscopic adrenalectomy using the LigaSure vessel sealing instead of using vascular clips or suturing. We report intraoperative findings, conversion rates, blood loss, operative time, morbidity, and postoperative outcomes.</p></sec><sec id="j_1905-7415.1102.545_s_004_w2aab3b7b1b1b6b1aab1c15b4Aa"><title style='display:none'>Results</title><p>Surgery was performed successfully for 18 patients. Surgery for the remaining 1 patient of the present case series was converted to open adrenalectomy because of surrounding tissue invasion. No mortality or major morbidity was observed. Estimated blood loss was a mean (range 20–300) 153 mL (excluding the loss in the patient whose surgery was converted to open adrenalectomy). The pathology showed 6 patients with potentially malignant PCC (Pheochromocytoma of the Adrenal Scaled Score (PASS) ≥ 4).</p></sec><sec id="j_1905-7415.1102.545_s_005_w2aab3b7b1b1b6b1aab1c15b5Aa"><title style='display:none'>Conclusions</title><p>The LigaSure device appears to be safe and effective in laparoscopic adrenalectomy for PCC. This clipless approach can be used with acceptable outcomes.</p></sec></abstract>ARTICLE2017-08-31T00:00:00.000+00:00TNF-α and G-CSF induce CD62L and CD106 expressions on rat bone marrow-derived MSCs<abstract><title style='display:none'>Abstract</title><p> Background: Accumulating evidence suggests that CD62L and CD 106 are positively expressed on the surface of mesenchymal stem cells (MSCs). It has been reported that both receptors can be induced by minor necrosis factor- ⃞ (TNF-α). granulocyte-colony stimulating factor (G-CSF). and vascular endothelial growth factor (VEGF) on leucocytes. However, whether these stimulations induce CD62L and CD 106 expressions on MSCs is still unknown. Thus, in the present study we investigated the effects of TNF-α. G-CSF and VEGF on CD62L and CD 160 expressions on the surface of MSCs.</p><p>Method: MSCs were isolated from rat bone marrows, and treated with different concentrations of TNF-α (0.1.1 and 10 ng/mL), G-CSF and VEGF (1.10. and 100 ng/mL) for 12 and 24 hours respectively. Then the expressions of CD62L and C'D 106 on the surface of MSCs were analyzed by flow cytometry.</p><p>Results: Immunochemistry assay showed positive CD90 but negative CD45 in the MSCs. Flow cytometry analysis suggested that TNF-α and G-CSF could induce CD62L and CD106 expressions on the surface of MSCs in a dose-dependent manner, but not in a time-dependent manner. Further, all the concentrations of VEGF had no significant effect on the CD62L and CD106 expressions.</p><p>Conclusion: CD62L and CD106 can be induced by TNF-α and G-CSF on the surface of MSCs. but not by VEGF. These findings can help improve BM-MSC migration capability and therapeutic efficiencies of MSC transplantation.</p></abstract>ARTICLE2017-02-04T00:00:00.000+00:00Percutaneous ipsilateral portal vein embolization using histoacryl glue: changing LR, resectibility rate and complications<abstract><title style='display:none'>Abstract</title><p> Background: Liver resection has been the main strategy for treating either primary or secondary liver cancer. However, major liver resection may lead to postoperative liver failure. Portal vein embolization (PVE) is a procedure to induce hypertrophy of a liver remnant (LR) before major resection surgery. There are many variations in procedural techniques, with different advantages and disadvantages.</p><p>Objective: We studied change in liver remnant volume, resectibility rate, and complications after percutaneous ipsilateral portal vein embolization (PVE) using histoacryl glue.</p><p>Methods: Clinical data of 25 patients who underwent ipsilateral PVE were reviewed. Eighteen patients who had pre- and post- CT studies had total liver volumes (TLV) and LR volumes determined before and after the procedure using MDCT volumetry. Complications and respectability rates were recorded.</p><p>Results: All 18 patients who had pre-CT and post-CT studies had increased LR volumes. The mean of LR volumes before and after ipsilateral PVE were calculated at about 449 ml and 586 ml, which were statistically significant (p &lt;0.001). The mean enlargement of LR was 30% (range 4 to 120%). There were no deaths or serious complications. The resectabilty rate was 76%.</p><p>Conclusion: Percutaneous transhepatic ipsilateral PVE could increase the LR volumes before major hepatic resection. There were no significant complications in our study group.</p></abstract>ARTICLE2017-02-04T00:00:00.000+00:00en-us-1