Sciendo Logo
  1. bookVolume 60 (2010): Issue 4 (December 2010)
Acta Pharmaceutica's Cover Image
Acta Pharmaceutica
Journal Details
License
Format
Journal
First Published
28 Feb 2007
Publication timeframe
4 times per year
Languages
English
access type Open Access

Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent

Pavagada Ramesh
Kanakapura Basavaiah
Nagaraju Rajendraprasad
Published Online: 17 Dec 2010
Page range: 445 - 454
Journal Details
License
Format
Journal
First Published
28 Feb 2007
Publication timeframe
4 times per year
Languages
English
Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent

A spectrophotometric method for the determination of doxycycline (DOX) is described. The method is based on the formation of blue colored chromogen due to reduction of tungstate and/or molybdate in Folin-Ciocalteu (F-C) reagent by DOX in alkaline medium. The colored species has an absorption maximum at 770 nm and the system obeys Beer's law over the concentration range 0.75-12.0 μg mL-1 DOX. The apparent molar absorptivity is 2.78 × 104 L mol-1 cm-1. The limit of quantification and detection values are reported to be 0.20 and 0.08 μg mL-1, respectively. Over the linear range applicable, the accuracy and precision of the method were evaluated on intra-day and inter-day basis. The reported mean accuracy value was 101.0 ± 1.7 %, the relative error was ≤ 2.7 % and the relative standard deviation was ≤ 2.5 %. Application of the proposed method to bulk powder and commercial pharmaceutical tablets is also presented. No significant difference was obtained between the results of the proposed method and the official BP method. The procedure described in this paper is simple, rapid, accurate and precise.

Keywords

  • doxycycline hyclate
  • Folin-Ciocalteu reagent
  • spectrophotometry

British Pharmacopoeia, Vol. II, Her Majesty's Stationery Office, London 1999, p. 1805.Search in Google Scholar

S. S. Mitic, G. Z. Miletic, D. A. Kostc, D. C. Naskovic-Djokic, B. B. Arsic and I. D. Rasic, A rapid and reliable determination of doxycycline hyclate by HPLC with UV detection in pharmaceutical samples, J. Serb. Chem. Soc. 73 (2008) 665-671; DOI: 10.2298/JSC0806665M.10.2298/JSC0806665MSearch in Google Scholar

D. Satinsky, L. M. L. Dos Santos, H. Sklenáøová, P. Solich, M. C. B. S. M. Montenegro, A. N. Araújo, Sequential injection chromatographic determination of ambroxol hydrochloride and doxycycline in pharmaceutical preparations, Talanta 68 (2005) 214-218; DOI: 10.1016/j.talanta.2005.06.059.10.1016/j.talanta.2005.06.059Search in Google Scholar

A. Van Schepdael, R. Kibaya, E. Roets and J. Hoogmartens, Analysis of doxycycline by capillary electrophoresis, Chromatographia 41 (1995) 367-369; DOI: 10.1007/BF02688053.10.1007/BF02688053Search in Google Scholar

J. L. Lopez Paz and J. M. Calatayud, Copper carbonate as a solid-bed reactor for spectrophotometric determination of doxycycline and oxytetracycline in an unsegmented continuous flow assembly, J. Pharm. Biomed. Anal. 11 (1993) 1093-1098; DOI: 10.1016/0731-7085(93)80087-H.10.1016/0731-7085(93)80087-HSearch in Google Scholar

Y. S. Chandra, V. S. Rao, P. S. R. Murthy, Y. Siva Chandra and V. Suryanarayana Rao, Determination of hostacycline and doxycycline using thorium(IV) as spectrophotometric reagent, Indian J. Pharm. Sci. 58 (1996) 157-159.Search in Google Scholar

M. S. Mahrous and M. M. Abdel-Khalek, Spectrophotometric determination of phenothiazines, tetracyclines and chloramphenicol with sodium cobaltinitrite, Talanta 31 (1984) 289-291; DOI: 10.1016/0039-9140(84)80281-7.10.1016/0039-9140(84)80281-7Search in Google Scholar

U. Saha, A. K. Sen, T. K. Das and S. K. Bhowal, Spectrophotometric determination of tetracyclines in pharmaceutical preparations, with uranyl acetate, Talanta 37 (1990) 1193-1196; DOI: 10.1016/0039-9140(90)80192-I.10.1016/0039-9140(90)80192-ISearch in Google Scholar

S. M. Sunaric, S. S. Mitic, G. Z. Miletic, A. N. Pavlovic and D. Naskovic-Djokic, Determination of doxycycline in pharmaceuticals based on its degradation by Cu(II)/H2O2 reagent in aqueous solution, J. Anal Chem. 64 (2009) 231-237; DOI: 10.1134/S1061934809030046.10.1134/S1061934809030046Search in Google Scholar

A. F. Espinosa-Mansilla, F. Salinas and I. De Orbe Paya, Simultaneous determination of sulfadiazine, doxycycline, furaltadone and trimethoprim by partial least squares multivariate calibration, Anal. Chim. Acta. 313 (1995) 103-112; DOI: 10.1016/0003-2670(95)00252-U.10.1016/0003-2670(95)00252-USearch in Google Scholar

N. Cicco, M. T. Lanorte, M. Paraggio, M. Viggiano and V. Lattanzio, A reproducible, rapid and inexpensive Folin-Ciocalteu micro-method in determining phenolics of plant methanol extracts, Microchem. J. 91 (2009) 107-110; DOI: 10.1016/j.microc.2008.08.01.Search in Google Scholar

N. P. Sadler and H. Jacobs, Application of the Folin-Ciocalteu reagent to the determination of salbutamol in pharmaceutical preparations, Talanta 42 (1995) 1385-1388; DOI: 10.1016/0039-9140(95)01534-I.10.1016/0039-9140(95)01534-ISearch in Google Scholar

C. S. P. Sastry and J. S. V. M. Lingeswara-Rao, Spectrophotometric methods for the determination of methotrexate in pharmaceutical formulations, Anal. Lett. 29 (1996) 1763-1768; DOI: 10.1080/00032719608001522.10.1080/00032719608001522Search in Google Scholar

International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2(R1), Complementary Guideline on Methodology dated 06 November 1996, incorporated in November 2005, London; http://www.ich.org/LOB/media/MEDIA417.pdfSearch in Google Scholar

G. L. Peterson, Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall, Anal. Biochem. 100 (1979) 201-220.10.1016/0003-2697(79)90222-7Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo