1. bookVolume 12 (2013): Issue 2 (December 2013)
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REPEATED-BATCH PRODUCTION OF LACCASE BY CERIPORIOPSIS SUBVERMISPORA

Published Online: 31 Dec 2013
Page range: 120 - 128
Journal Details
License
Format
Journal
First Published
28 Jun 2012
Publication timeframe
2 times per year
Languages
English
Copyright
© 2020 Sciendo

The aim of this study was to set parameters of repeated-batch cultivation of Ceriporiopsis subvermispora for laccase production and evaluate the efficiency of this type of cultivation for production of selected enzyme. The suitable conditions for repeated-batch cultivation were designed on the base of study of batch cultivation of white-rot fungus C. subvermispora. C. subvermispora was cultivated in media with different concentration of casein hydrolysate as nitrogen source and glucose as carbon source. A suitable concentration of casein hydrolysate to stimulate the laccase production was 1.5 and 2.5 g/L. Laccase production was started at certain critical concentration of glucose (5 g/L). In order to improve laccase production by repeated-batch cultivation of C. subvermispora, glucose was tested in concentration 10 g/L and casein hydrolysate in concentration 1.5 g/L. During a repeated-batch cultivation was measured increase laccase activities from 177.8 to 266 U/L. It was also observed, the cultivation time needed to reach maximum laccase production was shortened to 10 days.

Keywords

AQUIAR, A., SOUZA-CRUZ, P. B., FERRAZ, A.: Oxalic acid, Fe3+ - reduction activity and oxidative enzymes detected in culture extracts recovered from Pinustaeda wood chips biotreated by Ceriporiopsis subvermispora. Enzyme Microb.Tech., 38, 2006, 873-878.Search in Google Scholar

BIRHANLI, E., YESILADA, O.: Increased production of laccase by pellets of Funalia trogii ATCC 2008000 and Trametes versicolor ATCC 200801 in repeatedbatch mode. Enzyme Microb. Tech., 39, 2006, 1286-1293.Search in Google Scholar

BIRHANLI, E., YESILADA, O.: Enhanced production of laccase in repeated-batch cultures of Funalia trogii and Trametes versicolor. Biochem. Eng. J., 52, 2010, 33-37.Search in Google Scholar

CHMELOVÁ, D., ONDREJOVIČ, M., ONDÁŠ, V., ŠTURDÍK, E.: Influence of cultivation conditions on production of lignocellulolytic enzymes by Ceriporiopsissubvermispora. Biologia, 66, 2011, 748-754.Search in Google Scholar

EGGERT, C., TEMP, U., ERIKSSON, K. E.: The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase.Appl. Environ. Microbiol., 62, 1996, 1151-1158.Search in Google Scholar

FERRAZ, A., CÓRDOVA, A. M., MACHUCA, A.: Wood biodegradation and enzyme production by Ceriporiopsis subvermispora during solid-state fermentation of Eucalyptus grandis. Enzyme Microb. Tech., 32, 2003, 59-65.Search in Google Scholar

GALHAUP, C., WAGNER, H., HINTERSTOISSER, B., HALTRICH, D.: Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens.Enzyme Microb. Tech., 30, 2002, 529-536.Search in Google Scholar

HAMMEL, K. E., CULLEN, D.: Role of fungal peroxidases in biological ligninolysis.Curr. Opin. Plant Biol., 11, 2008, 349-355.Search in Google Scholar

HOWARD, R. L., ABOTSI, E., JANSEN VAN RENSBURG, E. L., HOWARD. S.: Lignocellulose biotechnology: issues of bioconversion and enzyme production.Afr. J. Biotechnol., 2, 2003, 602-619.Search in Google Scholar

KAAL, E. E. J., FIELD, J. A., JOYCE, T. W.: Increasing ligninolytic enzyme activities in several white-rot basidiomycetes by nitrogen-sufficient media.Bioresour. Technol., 53, 1995, 133-139.Search in Google Scholar

LEE, K. H., WI, S. G., SINGH, A. P., KIM, Y. S.: Micromorphological characteristics of decayed wood and laccase produced by the brown-rot fungus Coniophoraputeana. J. Wood Sci., 50, 2004, 281-284.Search in Google Scholar

LEVIN, L., HERRMANN, C., PAPINUTTI, V. L.: Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology. Biochem. Eng. J., 39, 2008, 207-214.Search in Google Scholar

LEVIN, L., MELIGNANI, E., RAMOS, A. M.: Effect of nitrogen sources and vitamins on lignolytic enzyme production by some white-rot fungi. Dye decolorization by selected culture filtrates. Bioresour. Technol., 101, 2010, 4554-4563.Search in Google Scholar

MILLER, G. L.: Use of dinitrosalicylic reagent for the determination of reducing sugar. Anal. Chem., 31, 1959, 426-428.Search in Google Scholar

PÉREZ, J., MARTÍNEZ, J., LA RUBIA, T.: Purification and partial characterization of a laccase from white rot fungus Phanerochaete flavido-alba. Appl. Environ.Microbiol., 62, 1996, 4263-4267. POZDNYAKOVA, N. N., RODAKIEWICZ-NOWAK, J., TURKOVSKAYA, O. V.: Catalytic properties of yellow laccase from Pleurotus ostreatus D1. J. Mol. Catal.B. Enzym., 30, 2004, 19-24.Search in Google Scholar

RÜTTIMANN, C., SCHWEMBER, E., SALAS, L., CULLEN, D., VICUNA, R.: Ligninolytic enzymes of the white rot basidiomycetes Phlebia brevispora and Ceriporiopsis subvermispora. Biotechnol. Appl. Biochem., 16,1992, 64-76.Search in Google Scholar

SHIN, T., MURAO, S., MATSUMURA, E.: A chromogenic oxidative coupling reaction of laccase: application for laccase and angiotensin I converting enzyme assay. Anal. Biochem., 166, 1987, 380-388.Search in Google Scholar

THIRUCHELVAM, A. T., RAMSAY, J. A.: Growth and laccase production kinetics of Trametes versicolor in a stirred tank reactor. Appl. Environ. Microbiol., 74, 2007, 547-554.Search in Google Scholar

TORRES, E., BUSTOS-JAIMES, I., LE BORGNE, S.: Potential use of oxidative enzymes for the detoxification of organic pollutants. Appl. Catal., B., 46, 2003, 1-15.Search in Google Scholar

WESENBERG, D., KYRIAKIDES, I., AGATHOS, N.: White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnol. Adv., 22, 2003, 161-187. Search in Google Scholar

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