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Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test

Marcin Mickiewicz

Marcin Mickiewicz

Division of Veterinary Epidemiology and Economics
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Mickiewicz, Marcin
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Zofia Nowek

Zofia Nowek

Division of Veterinary Epidemiology and Economics
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Nowek, Zofia
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Michał Czopowicz

Michał Czopowicz

Division of Veterinary Epidemiology and Economics
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Czopowicz, Michał
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Agata Moroz-Fik

Agata Moroz-Fik

Division of Veterinary Epidemiology and Economics
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Moroz-Fik, Agata
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Adrian-Valentin Potărniche

Adrian-Valentin Potărniche

Department of Infectious Diseases and Preventive Medicine, Law and Ethics, University of Agricultural Sciences and Veterinary MedicineCluj-Napoca, Romania
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Potărniche, Adrian-Valentin
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Kinga Biernacka

Kinga Biernacka

Division of Veterinary Epidemiology and Economics
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Biernacka, Kinga
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Olga Szaluś-Jordanow

Olga Szaluś-Jordanow

Department of Small Animal Diseases with Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences–SGGWWarsaw, Poland
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Szaluś-Jordanow, Olga
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Paweł Górski

Paweł Górski

Division of Parasitology and Invasiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences–SGGWWarsaw, Poland
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Górski, Paweł
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Alistair Antonopoulos

Alistair Antonopoulos

KreavetKruibeke, Belgium
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Antonopoulos, Alistair
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Iwona Markowska-Daniel

Iwona Markowska-Daniel

Division of Veterinary Epidemiology and Economics
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Markowska-Daniel, Iwona
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Marián Várady

Marián Várady

Institute of Parasitology, Slovak Academy of SciencesKošice, Slovakia
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Várady, Marián
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Jarosław Kaba

Jarosław Kaba

Division of Veterinary Epidemiology and Economics
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Kaba, Jarosław
  
Mar 25, 2025
Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test's Cover Image
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Published Online: Mar 25, 2025
Page range: 83 - 90
Received: Sep 12, 2024
Accepted: Mar 11, 2025
DOI: https://doi.org/10.2478/jvetres-2025-0016
Keywords
© 2025 Marcin Mickiewicz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1.

The observed percentage of unhatched eggs, larvae of the first and second stage (L1-L2) and larvae of the third stage (L3; percentage development) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Dots – arithmetic means; whiskers – 95% confidence intervals; asterisks (*) – statistically significantly lower percentages than in the control wells according to Dunnett’s test at α = 0.05
The observed percentage of unhatched eggs, larvae of the first and second stage (L1-L2) and larvae of the third stage (L3; percentage development) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Dots – arithmetic means; whiskers – 95% confidence intervals; asterisks (*) – statistically significantly lower percentages than in the control wells according to Dunnett’s test at α = 0.05

Fig. 2.

The five-parameter logistic curve (blue line) fitted to the observed percentage development of larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Red dots – exact values of tested concentrations indicated. Orange broken lines and arrows – DMSO concentrations inhibiting the development of 10%, 50% and 90% of L3
The five-parameter logistic curve (blue line) fitted to the observed percentage development of larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Red dots – exact values of tested concentrations indicated. Orange broken lines and arrows – DMSO concentrations inhibiting the development of 10%, 50% and 90% of L3

Fig. 3.

The proportion of developed larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. (out of 100 counted L3) at increasing dimethyl sulfoxide (DMSO) concentrations. P-values of McNemar’s test
The proportion of developed larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. (out of 100 counted L3) at increasing dimethyl sulfoxide (DMSO) concentrations. P-values of McNemar’s test

The observed percentage of eggs, larvae of the first and second stage (L1 and L2) and larvae of the third stage (L3; percentage development – PD) at increasing dimethyl sulfoxide (DMSO) concentrations

DMSO concentration Percentage of developmental stages observed after the 7-day incubation (%) a
n L3 (PD) Unhatched eggs L1 and L2
P-value b P-value b P-value b
0.0% (control) 30 95.5 ± 3.1 (87.5–100) -c 0.05 ± 0.28 (0–1.54) -c 4.43 ± 3.08 (0–12.5) -c
0.6% 30 97.0 ± 2.6 (89.2–100) 0.856 0.99 ± 1.60 (0–8.11) 0.022* 1.99 ± 2.26 (0–7.9) 0.450
1.3% 30 92.8 ± 4.4 (81.0–100) 0.335 0.85 ± 1.27 (0–4.11) 0.068 6.35 ± 4.40 (0–19.0) 0.683
2.6% 30 76.3 ± 8.8 (50.0–91.8) <0.001* 0.49 ± 0.90 (0–3.13) 0.566 23.2 ± 8.8 (8.2–50.0) <0.001*
5.2% 30 27.6 ± 10.4 (10.8–50.0) <0.001* 0.74 ± 1.43 (0–5.41) 0.157 71.7 ± 10.5 (50.0–89.2) <0.001*
10.4% 30 5.7 ± 6.6 (0–33.7) <0.001* 1.21 ± 1.53 (0–5.97) 0.003* 93.1 ± 6.6 (66.3–100) <0.001*
20.8% 30 0.04 ± 0.22 (0–1.2) <0.001* 0.84 ± 1.27 (0–4.62) 0.073 99.1 ± 1.3 (95.4–100) <0.001*
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