Open Access

Determination of tylosin concentration in sow’s milk after intramuscular administration

Piotr Cybulski

Piotr Cybulski

Goodvalley Agro S.A.Przechlewo, Poland
Orcid profile
Search for this author on
Sciendo|Google Scholar
Cybulski, Piotr
, 
Anna Gajda

Anna Gajda

Department of Pharmacology and Toxicology, National Veterinary Research InstitutePuławy, Poland
Orcid profile
Search for this author on
Sciendo|Google Scholar
Gajda, Anna
 and 
Aleksandra Kuśmierz

Aleksandra Kuśmierz

Department of Pharmacology and Toxicology, National Veterinary Research InstitutePuławy, Poland
Orcid profile
Search for this author on
Sciendo|Google Scholar
Kuśmierz, Aleksandra
  
Jul 15, 2025
Determination of tylosin concentration in sow’s milk after intramuscular administration's Cover Image
About this article
Previous Article
Next Article
Cite
Download Cover
Published Online: Jul 15, 2025
Received: Feb 12, 2025
Accepted: Jul 12, 2025
DOI: https://doi.org/10.2478/jvetres-2025-0008
Keywords
© 2025 Piotr Cybulski et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1.

Ion chromatograms of A – blank sow’s milk sample; B – milk sample fortified with tylosin (TYL) at 50 μg/L; C – milk sample at the concentration of 883 μg/L obtained one day after tylosin injection. cps – counts per s; IS – internal standard
Ion chromatograms of A – blank sow’s milk sample; B – milk sample fortified with tylosin (TYL) at 50 μg/L; C – milk sample at the concentration of 883 μg/L obtained one day after tylosin injection. cps – counts per s; IS – internal standard

Fig. 2.

The tylosin concentration–time profile in sow’s milk (n = 5) following treatment consisting of three injectable daily doses of 10 mg of the antibiotic per kg of body weight. SE – standard error; SD – standard deviation
The tylosin concentration–time profile in sow’s milk (n = 5) following treatment consisting of three injectable daily doses of 10 mg of the antibiotic per kg of body weight. SE – standard error; SD – standard deviation

Tylosin residues in milk samples (μg/L) after the intramuscular administration of the antibiotic

Sampling time (h) Goats (n = 5) (1) Goats (n = 24) (32) Ewes (n = 7) (26) Sows (n = 5) (this study)
0 -* -* 0* 0*
2 2,940 - -
3 - - - 1,802
4 1,730 - - -
6 700 - - -
8 390 - - -
10 160 - - -
12 0 - 632 -
24 - -* 128* 371*
36 - - 1,822 -
48 - -* 471* 482*
60 - - 1647 -
72 - 198 263* -
84 - - 1,046 -
96 - NS 161* -
108 - - 900 -
120 - NS 142 97
132 - - 31 -
144 - NS 0 -
156 - - - -
168 - 0 - 6

Validation parameters of the method for the analysis of tylosin in sow’s milk

Analyte Fortification level (μg/L) Repeatability (%) Reproducibility (%) Recovery (%)
Tylosin 5.0 9.2 13.8 98.2
50.0 7.0 6.7 105.8
100.0 4.7 4.1 100.6
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
Journal RSS Feed