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Biomarkers of oxidative stress, biochemical changes, and the activity of lysosomal enzymes in the livers of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against yersiniosis before a Yersinia ruckeri challenge

Natalia Kurhaluk

Natalia Kurhaluk

Department of Animal PhysiologySłupsk, Poland
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Kurhaluk, Natalia
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Joanna Grudniewska

Joanna Grudniewska

Department of Salmonid Research, Stanislaw Sakowicz Inland Fisheries InstituteRutki, Żukowo, Poland
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Grudniewska, Joanna
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Agnieszka Pękala-Safińska

Agnieszka Pękala-Safińska

Department of Preclinical Sciences and Infectious Diseases, Faculty of Veterinary Medicine and Animal Sciences, Poznań University of Life SciencesPoznań, Poland
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Pękala-Safińska, Agnieszka
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Joanna Pajdak-Czaus

Joanna Pajdak-Czaus

Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in OlsztynOlsztyn, Poland
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Pajdak-Czaus, Joanna
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Elżbieta Terech-Majewska

Elżbieta Terech-Majewska

Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in OlsztynOlsztyn, Poland
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Terech-Majewska, Elżbieta
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Aleksandra Platt-Samoraj

Aleksandra Platt-Samoraj

Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in OlsztynOlsztyn, Poland
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Platt-Samoraj, Aleksandra
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Halina Tkaczenko

Halina Tkaczenko

Department of Zoology, Institute of Biology, Pomeranian University in SłupskSłupsk, Poland
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Tkaczenko, Halina
  
Sep 23, 2024
Biomarkers of oxidative stress, biochemical changes, and the activity of lysosomal enzymes in the livers of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against yersiniosis before a Yersinia ruckeri challenge's Cover Image
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Published Online: Sep 23, 2024
Page range: 325 - 336
Received: Apr 06, 2024
Accepted: Sep 04, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0050
Keywords
© 2024 Natalia Kurhaluk et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Oxidative stress biomarkers estimated based on the levels of 2-thiobarbituric acid reactive substances (TBARS, A), aldehyde derivatives (B) and ketone derivatives (C) of oxidatively modified proteins, and total antioxidant capacity (D) in the liver tissue of rainbow trout orally immunised with Y. ruckeri vaccine and challenged with Y. ruckeri. Data are presented as means ± standard deviation (n = 10); * – significant differences (P-value < 0.05) between the untreated control group and the unvaccinated group challenged with Y. ruckeri; ** – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the vaccinated and unchallenged group; ## – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the unvaccinated group challenged with Y. ruckeri
Oxidative stress biomarkers estimated based on the levels of 2-thiobarbituric acid reactive substances (TBARS, A), aldehyde derivatives (B) and ketone derivatives (C) of oxidatively modified proteins, and total antioxidant capacity (D) in the liver tissue of rainbow trout orally immunised with Y. ruckeri vaccine and challenged with Y. ruckeri. Data are presented as means ± standard deviation (n = 10); * – significant differences (P-value < 0.05) between the untreated control group and the unvaccinated group challenged with Y. ruckeri; ** – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the vaccinated and unchallenged group; ## – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the unvaccinated group challenged with Y. ruckeri

Fig. 2.

Activity of the alanyl aminopeptidase (nmol·h-1·mg-1 protein), leucyl aminopeptidase (nmol·h-1·mg-1 protein), acid phosphatase (AcP, nmol·h-1·mg-1 protein), and β-N-acetylglucosaminidase (nmol·h-1·mg-1 protein) in the liver tissue of rainbow trout orally immunised against Y. ruckeri and challenged with this bacterium. Data are presented as means ± standard deviation (n = 10); * – significant differences (P-value < 0.05) between the untreated control group and the unvaccinated group challenged with Y. ruckeri; ** – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the vaccinated and unchallenged group; # – significant differences (P-value < 0.05) between the untreated control group and the vaccinated and unchallenged group; ## – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the unvaccinated group challenged with Y. ruckeri
Activity of the alanyl aminopeptidase (nmol·h-1·mg-1 protein), leucyl aminopeptidase (nmol·h-1·mg-1 protein), acid phosphatase (AcP, nmol·h-1·mg-1 protein), and β-N-acetylglucosaminidase (nmol·h-1·mg-1 protein) in the liver tissue of rainbow trout orally immunised against Y. ruckeri and challenged with this bacterium. Data are presented as means ± standard deviation (n = 10); * – significant differences (P-value < 0.05) between the untreated control group and the unvaccinated group challenged with Y. ruckeri; ** – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the vaccinated and unchallenged group; # – significant differences (P-value < 0.05) between the untreated control group and the vaccinated and unchallenged group; ## – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the unvaccinated group challenged with Y. ruckeri

Activity of superoxide dismutase (SOD, U·mg-1 protein), catalase (CAT, nmol·min-1·mg-1 protein), glutathione reductase (GR, nmol·min-1·mg-1 protein) and glutathione peroxidase (GPx, nmol·min-1·mg-1 protein) antioxidant enzymes in the liver tissue of rainbow trout orally vaccinated against Yersinia ruckeri and challenged with this bacterium

Parameter Untreated control group Vaccinated unchallenged group Unvaccinated group challenged with Yersinia ruckeri Vaccinated group challenged with Yersinia ruckeri
SOD, U·mg-1 protein 643.90 ± 99.08* 627.65 ± 38.09 921.76 ± 139.25* ## 656.44 ± 49.11##
CAT, nmol·min-1·mg-1 protein 12.92 ± 0.67* # 9.93 ± 0.59# ** 22.08 ± 1.88* ## 11.52 ± 0.98** ##
GR, nmol·min-1·mg-1 protein 15.19 ± 1.84* # 25.13 ± 2.17# 11.27 ± 3.70* ## 24.19 ± 2.12##
GPx, nmol·min-1·mg-1 protein 238.89 ± 23.26 250.61 ± 43.82** 186.96 ± 46.39## 357.86 ± 70.71** ##

Activity of alanine aminotransferase (ALT, nmol·min-1·mg-1 protein) and aspartate aminotransferase (AST, nmol·min-1·mg-1 protein), AST/ALT ratio, lactate dehydrogenase activity (LDH, nmol·min-1·mg-1 protein), lactate and pyruvate levels (nmol·mg-1 protein) and the L/P ratio in the liver tissue of rainbow trout orally immunised against Yersinia ruckeri and challenged with this bacterium

Parameter Group
Untreated control group Vaccinated unchallenged group Unvaccinated group challenged with Yersinia ruckeri Vaccinated group challenged with Yersinia ruckeri
ALT, nmol·min-1·mg-1 protein 4.46 ± 0.48* 4.25 ± 0.72 5.67 ± 0.65* ## 4.21 ± 0.68##
AST, nmol·min-1·mg-1 protein 3.36 ± 0.23* 3.48 ± 0.34 4.12 ± 0.33* ## 3.26 ± 0.33##
AST/ALT ratio 0.75 ± 0.06 0.83 ± 0.11 0.76 ± 0.10 0.78 ± 0.09
LDH, nmol·min-1·mg-1 protein 22.61 ± 0.81 21.87 ± 0.21 22.09 ± 0.42 22.20 ± 0.33
Lactate, nmol·mg-1 protein 63.50 ± 17.12* 74.61 ± 19.64 102.97 ± 18.09* ## 64.94 ± 11.78##
Pyruvate, nmol·mg-1 protein 29.56 ± 6.13* 37.01 ± 5.96 45.37 ± 11.38* ## 33.31 ± 5.50##
L/P ratio 2.22 ± 0.70 2.07 ± 0.64 2.39 ± 0.91 1.99 ± 0.49
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Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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