Open Access

Porcine epidemic diarrhoea virus induces cell-cycle arrest through the DNA damage-signalling pathway

Yi-Ran Luo

Yi-Ran Luo

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China
Search for this author on
Sciendo|Google Scholar
Luo, Yi-Ran
, 
Shu-Ting Zhou

Shu-Ting Zhou

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China
Search for this author on
Sciendo|Google Scholar
Zhou, Shu-Ting
, 
Liang Yang

Liang Yang

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China
Search for this author on
Sciendo|Google Scholar
Yang, Liang
, 
Yuan-Ping Liu

Yuan-Ping Liu

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China
Search for this author on
Sciendo|Google Scholar
Liu, Yuan-Ping
, 
Sheng-Yao Jiang

Sheng-Yao Jiang

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China
Search for this author on
Sciendo|Google Scholar
Jiang, Sheng-Yao
, 
Yeliboli Dawuli

Yeliboli Dawuli

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China
Search for this author on
Sciendo|Google Scholar
Dawuli, Yeliboli
, 
Yi-Xuan Hou

Yi-Xuan Hou

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China
Search for this author on
Sciendo|Google Scholar
Hou, Yi-Xuan
, 
Tian-Xing Zhou

Tian-Xing Zhou

College of Biological Science and Technology, Beijing Forestry UniversityBeijing, China
Search for this author on
Sciendo|Google Scholar
Zhou, Tian-Xing
 and 
Zhi-Biao Yang

Zhi-Biao Yang

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China
Search for this author on
Sciendo|Google Scholar
Yang, Zhi-Biao
  
Mar 24, 2020
Porcine epidemic diarrhoea virus induces cell-cycle arrest through the DNA damage-signalling pathway's Cover Image
About this article
Previous Article
Next Article
Cite
Download Cover
Published Online: Mar 24, 2020
Page range: 25 - 32
Received: Jun 07, 2019
Accepted: Mar 09, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0024
Keywords
© 2020 Y.R. Luo et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

A – analysis workflow for flow cytometry data; B – changes in cell cycle of Vero cells infected with PEDV between 0 h and 32 h after infection
A – analysis workflow for flow cytometry data; B – changes in cell cycle of Vero cells infected with PEDV between 0 h and 32 h after infection

Fig. 2

changes in proportion of cells in the G1/G0 phase of Vero cells infected with PEDV between 0 h and 32 h after infection
changes in proportion of cells in the G1/G0 phase of Vero cells infected with PEDV between 0 h and 32 h after infection

Fig. 3

Changes in proportion of cells in the S phase of Vero cells infected with PEDV between 0 h and 32 h after infection
Changes in proportion of cells in the S phase of Vero cells infected with PEDV between 0 h and 32 h after infection

Fig. 4

Changes in proportion of cells in the G2/M phase of Vero cells infected with PEDV between 0 h and 32 h after infection
Changes in proportion of cells in the G2/M phase of Vero cells infected with PEDV between 0 h and 32 h after infection

Fig. 5

Changes over time in Cyclin B1, D1, and E1 in Vero cells infected with PEDV (M – control; I – PEDV infected cells)
Changes over time in Cyclin B1, D1, and E1 in Vero cells infected with PEDV (M – control; I – PEDV infected cells)

Fig. 6

Changes over time in Chk.2, H2A.X, p53, and PEDV-N in Vero cells infected with PEDV (M – control, I – PEDV infected cells)
Changes over time in Chk.2, H2A.X, p53, and PEDV-N in Vero cells infected with PEDV (M – control, I – PEDV infected cells)

Fig. 7

Cell cycle data of control or PEDV-infected Vero cells treated by DMSO, ATM inhibitor, or/ and Chk.2 inhibitor
Cell cycle data of control or PEDV-infected Vero cells treated by DMSO, ATM inhibitor, or/ and Chk.2 inhibitor

Fig. 8

Virus proliferation evaluated by real-time PCR of control or PEDV infected Vero cells treated by DMSO, ATM inhibitor or Chk.2 inhibitor
Virus proliferation evaluated by real-time PCR of control or PEDV infected Vero cells treated by DMSO, ATM inhibitor or Chk.2 inhibitor
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
Journal RSS Feed