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Contributions to Tobacco & Nicotine Research

Contributions to Tob. Res.

<abstract><title style='display:none'>Abstract</title> This paper describes a method for the extraction, separation and determination of amino acids, both free and protein-bound, which are present in tobacco. The introduction of the lyophilization process and the ''cold finger'' technique made it possible to obtain a good purification of extracts which, unlike the previous methods, have been preserved for a long time. The combined use of column chromatography and mass spectrometry enabled all amino acids present to be detected. The analyses carried out on different tobacco varieties showed certain quantitative and qualitative differences in the composition of amino acids and only small quantitative variations in the composition of protein-bound amino acids</abstract>

Abstract

Monopoli di Stato, Laboratorio Chimico Centrale, Rome, Italy

<article-title>Quantitative Determination of Free and Protein-Bound Amino Acids of Tobacco</article-title>

Quantitative Determination of Free and Protein-Bound Amino Acids of Tobacco

{"article-title":"Quantitative Determination of Free and Protein-Bound Amino Acids of Tobacco"}

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Advisory Board　　　 Bernhard Eitzinger, delfortgroup AG, Traun, Austria Ernest Groman, University of Vienna, Vienna, Austria Daniel Heck, HeckTox LLC, Greensboro, NC, USA Anne Fisher, University of Kentucky, Lexington, KY, USA Xie Jianping, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China Hubert Klus, St. Pölten, Austria John H. Lauterbach, Lauterbach &amp; Associates LLC, Macon, GA, USA Ramsey Lewis, North Carolina State University, Raleigh, NC, USA Chuan Liu, Southampton, UK Tryggve Ljung, Swedish Match, Stockholm, Sweden Michael W. Ogden, Ogden Ology, LLC, Lewisville, NC, USA Thilo Paschke, Geneva, Switzerland Stephen W. Purkis, Bristol, UK Elmar Richter †, Ludwig-Maximilians-University, Munich, Germany Wolfram Roeper, Hamburg, Germany Wolfgang Trinkies, Bayreuth, Germany Andreas Zemann, Leopold-Franzens-University of Innsbruck, Innsbruck, Austria Editors Wolf-Dieter Heller Gerhard Scherer Editorial Office Barbara Boenke Brigitte Müller Anja Thielen Contact <A href="mailto:editors@beitraege-bti.de">editors@beitraege-bti.de</A> Publisher De Gruyter Poland Bogumiła Zuga 32A Str. 01-811 Warsaw, Poland T: +48 22 701 50 15

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Contributions to Tobacco and Nicotine Research (formerly: Beiträge zur Tabakforschung International / Contribution to Tobacco Research International) publishes articles with a focus on tobacco, nicotine, and related consumer products. In addition, all aspects of research on the tobacco plant as well as on product technology and manufacturing are relevant for the Journal. Products of interest comprise (i) combustible tobacco products (cigarettes, cigars, pipes, etc.), (ii) smokeless tobacco (SLT) products (snuff, snus, chewing tobacco, etc.), (iii) tobacco heating products (THP), (iv) electronic cigarettes (e-cigs, w/o nicotine), (v) oral nicotine delivery (OND) products. All scientific investigations on product-related properties (including physical, chemical, biological, and toxicological properties) as well as on the influence on consumers’ behavior and response (including acute and long-term product use patterns and biomarker profiles) are considered for publication. Submissions can be of the following types: original investigations, reviews, short reports commentaries, letters to the editor. Fields covered by the Journal <UL> <LI>Research into the tobacco plant </LI> <UL> <LI>Tobacco plant </LI> <LI>Metabolism </LI> <LI>Cultivation </LI> <LI>Pesticides Biotechnology (Plant pathology, Mycology, Microbiology) </LI> </UL></UL> <UL> <LI>Manufacture of tobacco products </LI> <UL> <LI>Processing of tobacco </LI> <LI>Flavor </LI> <LI>Manufacture of cigarettes and other products </LI> <LI>Tobacco and environment </LI> <LI>Chemical engineering including physical chemistry </LI> </UL></UL> <UL> <LI>Analysis and properties of tobacco smoke </LI> <UL> <LI>Physical properties </LI> <LI>Chemical properties </LI> <LI>Combustion studies </LI> <LI>Mainstream/sidestream smoke </LI> <LI>Filter studies </LI> <LI>Special substances </LI> <LI>Analytical methods </LI> <LI>Machine smoking </LI> </UL></UL> <UL> <LI>Tobacco products other than cigarettes </LI> <UL> <LI>Cigars / cigarillos </LI> <LI>Pipe tobacco </LI> <LI>Smokeless tobacco (SLT) products (snuff, snus, chewing tobacco) </LI></UL> <LI>New products </LI> <UL> <LI>Tobacco heating products (THP) </LI> <LI>Electronic cigarettes (e-cigs, w/o nicotine) </LI> <LI>Oral nicotine devices (OND, e.g., nicotine pouches) </LI> </UL></UL> <UL> <LI>Human smoking behavior </LI> <UL> <LI>Biomonitoring </LI> <LI>Consumer behavior </LI></UL></UL> Open Access Statement The journal is an Open Access journal that allows a free unlimited access to all its contents without any restrictions upon publication to all users. Archiving Sciendo archives the contents of this journal in <A href="https://www.portico.org/">Portico</A> - digital long-term preservation service of scholarly books, journals and collections. Plagiarism Policy The editorial board is participating in a growing community of <A href="https://www.crossref.org/services/similarity-check/">Similarity Check System's</A> users in order to ensure that the content published is original and trustworthy. Similarity Check is a medium that allows for comprehensive manuscripts screening, aimed to eliminate plagiarism and provide a high standard and quality peer-review process.

Contributions to Tobacco & Nicotine Research

Quantitative Determination of Free and Protein-Bound Amino Acids of Tobacco

N. Carugno,
N. Carugno,
M. Neri and
M. Neri and
G. Lionetti
G. Lionetti

Published Online: 26 Jul 2014

Volume & Issue: Volume 7 (1974) - Issue 4 (April 1974)

Page range: 222 - 227

Received: 09 Jul 1973

Journal & Issue Details

Contributions to Tobacco & Nicotine Research

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References

Recent Articles

Contributions to Tobacco & Nicotine Research

Quantitative Determination of Free and Protein-Bound Amino Acids of Tobacco

N. Carugno,N. Carugno,M. Neri andM. Neri andG. LionettiG. Lionetti

Published Online: 26 Jul 2014

Volume & Issue: Volume 7 (1974) - Issue 4 (April 1974)

Page range: 222 - 227

Received: 09 Jul 1973

Journal & Issue Details

Contributions to Tobacco & Nicotine Research

PDF Preview

References

Recent Articles

N. Carugno,
N. Carugno,
M. Neri and
M. Neri and
G. Lionetti
G. Lionetti