1. bookVolume 5 (2021): Issue 1 (January 2021)
Journal Details
First Published
16 Jun 2017
Publication timeframe
1 time per year
access type Open Access

Isolation and Characterization of a New Streptomyces strain LG10 from an Unexploited Algerian Saharan Atlas

Published Online: 03 Jul 2021
Volume & Issue: Volume 5 (2021) - Issue 1 (January 2021)
Page range: 36 - 45
Received: 01 Mar 2021
Accepted: 01 Jun 2021
Journal Details
First Published
16 Jun 2017
Publication timeframe
1 time per year

An actinobacterial strain named LG10 was isolated from a Saharan Atlas soil (Laghouat, Algeria). The aerial hyphae were yellowish-white on all culture media with rectiflexibiles spore chains, suggested that this bacterium attached to Streptomyces. Furthermore, LG10 contained chemical characteristics that were diagnostic for the genus Streptomyces, such as the presence of LL-diaminopimelic acid isomer (LL-DAP) and glycine amino acid. The hydrolysates of whole-cell included non-characteristic sugars. Comparative analysis of the 16S rDNA sequence displayed a similarity level of 100% with Streptomyces puniceus NRRL ISP-5058T. The antimicrobial activity of the LG10 strain was better in the culture medium MB5. Streptomyces strains are good sources of bioactive compounds with multiple biological activities.


1. Solecka, J., Zajko, J., Postek, M., Rajnisz, A. (2012). Biologically active secondary metabolites from actinomycetes. Central EuropeanJournal of Biology, 7, 373-390. https://doi.org/10.2478/s11535-012-0036-110.2478/s11535-012-0036-1 Search in Google Scholar

2. Barka, E.A, Vatsa, P., Sanchez, L., Gaveau-Vaillant, N., Jacquard, C., Klenk, H.P., Clément, C., Ouhdouch, Y., Van Wezel, G.P. (2016). Taxonomy, physiology, and natural products of actinobacteria. Microbiology and Molecular Biology Reviews.80,1-43. https://doi.org/10.1128/MMBR.00019-1510.1128/MMBR.00019-15 Search in Google Scholar

3. Meklat, A., Bouras, N., Zitouni, A., Mathieu, F., Lebrihi, A., Schumann, P., Spröer, C., Klenk, H-P., Sabaou N. (2013). Actinopolysporarighensis sp. nov., a novel halophilicactinomycete isolated from Saharan soil in Algeria. Antonie van Leeuwenhoek, 104, 301-307. https://doi.org/10.1007/s10482-013-9948-710.1007/s10482-013-9948-7 Search in Google Scholar

4. Saker, R., Bouras, N., Zitouni, A., Ghoul, M., Rhode, M., Schumann, P., Spröer, C., Sabaou, N., Klenk, H-P. (2014). Mzabimycesalgeriensis gen. nov., sp. nov., a halophilic filamentous actinobacterium isolated from Saharan soil, and proposal of Mzabimycetaceae fam. nov. Antonie van leeuwenhoek, 106, 1021-1030.10.1007/s10482-014-0271-8 Search in Google Scholar

5. Khebizi, N., Boudjella, H., Bijani, C., Bouras, N., Klenk, H-P., Pont, F., Mathieu, F., Sabaou, N. (2018). Oligomycins A and E, major bioactive secondary metabolites produced by Streptomyces sp. strain HG 29 isolated from a Saharan soil. Journal of Medical Mycology, 28, 150-160. https://doi.org/10.1016/j.mycmed.2017.10.00710.1016/j.mycmed.2017.10.007 Search in Google Scholar

6. Tata, S., Aouiche, A., Bijani, C., Bouras, N., Pont, F., Mathieu, F., Sabaou, N. (2019). Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophan. Saudi Pharmaceutical Journal, 907-913. https://doi.org/10.1016/j.jsps.2019.06.00410.1016/j.jsps.2019.06.004 Search in Google Scholar

7. Hayakawa, M., &Nonomura. H. (1987). Humic acidvitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology, 65, 501-9. https://doi.org/10.1016/0385-6380(87)90108-710.1016/0385-6380(87)90108-7 Search in Google Scholar

8. Shirling, E.B, &Gottlieb, D. (1966). Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology, 13, 313-340. https://doi.org/10.1099/00207713-16-3-31310.1099/00207713-16-3-313 Search in Google Scholar

9. Waksman, S.A., (1961). The Actinomycetes. Classification, identification and descriptions of genera and species, vol. 2. Williams and Wilkins Co: Baltimore. Search in Google Scholar

10. Becker, B., Lechevalier, M.P., Gordon, R.E. &Lechevalier, H.A. (1964) Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Applied Microbiology, 12, 421–423. https://doi.org/10.1128/am.12.5.421-423.196410.1128/am.12.5.421-423.1964105814814215972 Search in Google Scholar

11. Lechevalier, M.P. &Lechevalier, H.A. (1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. International Journal of Systematic Bacteriology, 20, 435-443. https://doi.org/10.1099/00207713-20-4-43510.1099/00207713-20-4-435 Search in Google Scholar

12. Minnikin, D.E., Patel, P.V., Alshamaony, L., &Goodfellow, M. (1977). Polar lipid composition in the classification of Nocardia and related bacteria. International Journal of Systematic Bacteriology, 27, 104-117. https://doi.org/10.1099/00207713-27-2-10410.1099/00207713-27-2-104 Search in Google Scholar

13. Locci, R. (1989). Streptomycetes and related genera. In: Williams, S.T.; Sharpe, M.E.; Holt, J.G.; editors. Bergey’s manual of systematic bacteriology, vol. 4. Baltimore: Williams and Wilkins, P, 2451-92. Search in Google Scholar

14. Liu, D., Coloe, S., Baird, R., & Pedersen, J. (2000). Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology, 38, 471. https://doi.org/10.1128/jcm.38.1.471-471.200010.1128/JCM.38.1.471-471.2000 Search in Google Scholar

15. Belghit, S., Driche, E. H., Bijani, C., Zitouni, A., Sabaou, N., Badji, B., Mathieu, F. (2016). Activity of 2, 4–Di–tert–butylphenol produced by a strain of Streptomyces mutabilis isolated from a Saharan soil against Candida albicans and other pathogenic fungi. Journal of medical mycology, 26(2), 160–169. https://doi.org/10.1016/j.mycmed.2016.03.00110.1016/j.mycmed.2016.03.001 Search in Google Scholar

16. Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M. Na, H.Park, S. C., Jeon, Y.S., Lee, J. H., Yi, H., Won, S., Chun, J. (2012). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62, 716-21. https://doi.org/10.1099/ijs.0.038075-010.1099/ijs.0.038075-0 Search in Google Scholar

17. Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725-9. https://doi.org/10.1093/molbev/mst19710.1093/molbev/mst197 Search in Google Scholar

18. Lu, Q. P., Ye, J.J., Huang, Y.M., Liu, D., Liu, L.F., Dong, K., Razumova, E.A., Osterman, I.A., Sergiev, P.V., Dontsova, O.A., Jia, S.H., Huang, D.L & Sun, C.H. (2019). Exploitation of Potentially New Antibiotics from Mangrove Actinobacteria in Maowei Sea by Combination of Multiple Discovery Strategies. Antibiotics, 8 (4), 236. https://doi.org/10.3390/antibiotics804023610.3390/antibiotics8040236 Search in Google Scholar

19. El Karkouri, A., Ait Assou, S & El Hassouni, M. (2019). Isolation and screening of actinomycetes producing antimicrobial substances from an extreme Moroccan biotope. Pan African Medical Journal, 33, 329. https://dx.doi.org/10.11604%2Fpamj.2019.33.329.1901810.11604/pamj.2019.33.329.19018 Search in Google Scholar

20. Lechevalier H.A.&Lechevalier, M.P. (1970). Composition of whole-cell hydrolysates as a criterion in the classification of aerobic actinomycetes. In: Prauser, H., editor. The actinomycetales. VEB Gustav Fischer Verlag, Jena, p, 393-405. Search in Google Scholar

21. Lechevalier, M.P., de Bièvre, C., &Lechevalier, H.A. (1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochemical Systematics and Ecology, 5, 249-60. https://doi.org/10.1016/0305-1978(77)90021-710.1016/0305-1978(77)90021-7 Search in Google Scholar

22. Euzeby, J.P. (2021) List of bacterial names with standing in nomenclature-genus Streptomyces; 2020, http://www.bacterio.net/streptomycesa.html. Accessed March, 14 2021. Search in Google Scholar

23. Routien, J.B., & Hofmann, A. (1951). Streptomyces californicus productor de viomicina. Antibiotics and Chemotherapy, 1,387-389. Search in Google Scholar

24. Whitman, W.B., Goodfellow, M., Kämpfer, P., Busse H.J., Trujillo M.E., Ludwig, W., Suzuki K,i. (2012). Bergey’s Manual of Systematic Bacteriology - Parte A (Eds) vol 5: The Actinobacteria, 2nd edition. Springer, New York. p. 1715. Search in Google Scholar

25. Tan, L.T., Chan, KG., Pusparajah, P. Yin, W.F., Khan, T. M., Lee, L.H., Goh, B.H. (2019). Mangrove derived Streptomyces sp. MUM265 as a potential source of antioxidant and anticolon-cancer agents. BMC Microbiology, 19, 38. https://doi.org/10.1186/s12866-019-1409-710.1186/s12866-019-1409-7637522230760201 Search in Google Scholar

26. Kämpfer, P. (2012c). Genus Streptomyces. In Bergey’s Manual of Systematic Bacteriology, 2nd edn., vol. 5, The actinobacteria (edited by Goodfellow, Kämpfer, Busse, Trujillo, Suzuki, Ludwig and Whitman). Springer, New York, pp. 1455–1804. Search in Google Scholar

27. Saker, R., Bouras, N., Meklat, A., Zitouni, A., Schumann, P., Sproer, C., Sabaou, N., Klenk, H.P. (2015). Prauserella isguenensis sp. nov., a halophilic actinomycete isolated from desert soil. International Journal of Systematic and Evolutionary Microbiology, 65, 1598-1603. https://doi.org/10.1099/ijs.0.00014510.1099/ijs.0.00014525713039 Search in Google Scholar

28. Boubetra, D., Zitouni, A., Bouras, N., Schumann, P., Sproer, C., Klenk, H.P., Sabaou, N. (2015). Saccharothrix tamanrassetensis sp. nov., an actinomycete isolated from Saharan soil. International Journal of Systematic and Evolutionary Microbiology, 65, 1316-1320. https://doi.org/10.1099/ijs.0.00010410.1099/ijs.0.000104 Search in Google Scholar

29. Aouiche, A., Bouras, N., Mokrane, S., Zitouni, A., Schumann, P., Sproer, C., Sabaou, N., Klenk, H.P. (2015). Actinokineospora mzabensis sp. nov., a novel actinomycete isolated from Saharan soil. Antonie Van Leeuwenhoek, 107, 291-296. https://doi.org/10.1007/s10482-014-0328-810.1007/s10482-014-0328-8 Search in Google Scholar

30. Bouras, N., Meklat, A., Zitouni, A., Mathieu, F., Schumann, P., Sproer, C., Sabaou, N., Klenk, H.P. (2015). Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. Antonie Van Leeuwenhoek, 107, 313-320. https://doi.org/10.1007/s10482-014-0329-710.1007/s10482-014-0329-7 Search in Google Scholar

31. Meklat, A., Bouras, N., Zitouni, A., Sabaou, N., Mathieu F, Schumann P, Sproer C, Klenk HP. (2014). Saccharopolyspora ghardaiensis sp. nov., an extremely halophilicactinomycete isolated from Algerian Saharan soil. Journal of Antibiotics (Tokyo), 67:299-303. https://doi.org/10.1038/ja.2013.13610.1038/ja.2013.136 Search in Google Scholar

32. Moshtaghi Nikou, M., Ramezani, M., Harirchi, S., Makzoom, S., Amoozegar, M.A., Shahzadeh Fazeli, S.A, Schumann, P., Ventosa, A. (2017). Salinifilum gen. nov., with description of Salinifilum proteinilyticum sp. nov., an extremely halophilic actinomycete isolated from Meighan wetland, Iran, and reclassification of Saccharopolyspora aidingensis as Salinifilum aidingensis comb. nov. and Saccharopolyspora ghardaiensis as Salinifilum ghardaiensis comb. nov. International Journal of Systematic and Evolutionary Microbiology, 67, 4221-4227. https://doi.org/10.1099/ijsem.0.00228610.1099/ijsem.0.002286 Search in Google Scholar

33. Chaabanechaouch, F., Bouras, N., Mokrane, S., Zitouni, A., Schumann, P., Sproer, C., Sabaou, N., Klenk, H.P. (2016). Streptosporangium saharense sp. nov., an actinobacterium isolated from Saharan soil. International Journal of Systematic and Evolutionary Microbiology, 66,1371-1376. https://doi.org/10.1099/ijsem.0.00089010.1099/ijsem.0.000890 Search in Google Scholar

34. Lai, H., Jiang, Y., Saker, R., Chen, X., Bouras, N., Klenk, H.P., Wei, X., Jiang, Y., Sabaou, N. (2017). Reclassification of Mzabimyces algeriensis Saker et al. 2015 as Halopolyspora algeriensis comb. nov. International Journal of Systematic and Evolutionary Microbiology, 67, 2787-2790. https://doi.org/10.1099/ijsem.0.00202210.1099/ijsem.0.002022 Search in Google Scholar

35. Meklat, A., Bouras, N., Mokrane, S., Zitouni, A., Schumann, P., Sproer, C., Klenk, H.P., Sabaou, N. (2015). Bounagaea algeriensis gen. nov., sp. nov., an extremely halophilic actinobacterium isolated from a Saharan soil of Algeria. Antonie Van Leeuwenhoek, 108,473-482. https://doi.org/10.1007/s10482-015-0500-910.1007/s10482-015-0500-9 Search in Google Scholar

36. Djaballah, C.E., Kitouni, M., Raoult, D., Khelaifia, S. (2018), “Streptomyces massilialgeriensis” sp. nov., a new bacterial species isolated from an extremely saline soil collected from the dry lake of Ank el Djamel in Algeria. New Microbes and New Infection, 21,18-19. https://doi.org/10.1016/j.nmni.2017.10.00310.1016/j.nmni.2017.10.003 Search in Google Scholar

37. Martín J, F., Demain A, L. (1980). Control of antibiotic biosynthesis. Microbiological reviews, 44 (2): 230. Search in Google Scholar

38. Sanchez, S., Demain, A.L. (2002) Metabolic regulation of fermentation processes. Enzyme and Microbial Technology, 31, 895-906. https://doi.org/10.1016/S0141-0229(02)00172-210.1016/S0141-0229(02)00172-2 Search in Google Scholar

39. Aouiche, A., Bijani, C., Zitouni, A., Mathieu, F., Sabaou, N. (2014). Antimicrobial activity of saquayamycins produced by Streptomyces spp. PAL114 isolated from a Saharan soil. Journal of Medical Mycology, 24(2), 17–23. https://doi.org/10.1016/j.mycmed.2013.09.00110.1016/j.mycmed.2013.09.00124139182 Search in Google Scholar

40. Aouiche, A., Meklat, A., Bijani, C., Zitouni, A., Sabaou, N., Mathieu, F. (2015). Production of vineomycin A1 and chaetoglobosin A by Streptomyces sp. PAL114. Annals of Microbiology, 65 (3), 1351–1359. https://doi.org/10.1007/s13213-014-0973-110.1007/s13213-014-0973-1 Search in Google Scholar

41. Driche, E. H., Belghit, S., Bijani, C., Zitouni, A., Sabaou, N., Mathieu, F., Badji, B. (2015). A new Streptomyces strain isolated from Saharan soil produces di–(2–ethylhexyl) phthalate, a metabolite active against methicillin–resistant Staphylococcus aureus. Annals of Microbiology, 65(3), 1341–1350.10.1007/s13213-014-0972-2 Search in Google Scholar

42. Driche, E. H., Sabaou, N., Bijani, C., Zitouni, A., Pont, F., Mathieu, F., Badji, B. (2017). Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug–resistant Staphylococcus aureus. World Journal of Microbiology and Biotechnology, 33(6), 105. https://doi.org/10.1007/s11274-017-2265-y10.1007/s11274-017-2265-y28466299 Search in Google Scholar

43. Toumatia, O., Yekkour, A., Goudjal, Y., Riba, A., Coppel, Y., Mathieu, F., Sabaou, N., Zitouni, A. (2015). Antifungal properties of an actinomycin D- producing strain, Streptomyces sp. IA1, isolated from a Saharan soil. Journal of Basic Microbiology, 55(2), 221–228. https://doi.org/10.1002/jobm.20140020210.1002/jobm.20140020225284744 Search in Google Scholar

44. Djinni, I., Defant, A., Djoudi, W., Chaouch, F. C., Souagui, S., Kecha, M., Mancini, I. (2019). Modeling improved production of the chemotherapeutic polypeptide actinomycin D by a novel Streptomyces sp. strain from a Saharan soil. Heliyon, 5(5), 01695. https://doi.org/10.1016/j.heliyon.2019.e0169510.1016/j.heliyon.2019.e01695653896931193702 Search in Google Scholar

45. Yekkour, A., Meklat, A., Bijani, C., Toumatia, O., Errakhi, R., Lebrihi, A., Mathieu, F., Sabaou, N. (2015). A novel hydroxamic acid-containing antibiotic produced by a Saharan soil-living Streptomyces strain. Letters in applied microbiology, 60(6), 589–596. https://doi.org/10.1111/lam.1241210.1111/lam.1241225754683 Search in Google Scholar

46. Djinni, I., Djoudi, W., Souagui, S., Rabia, F., Rahmouni, S., Mancini, I., Kecha, M. (2018). Streptomyces thermoviolaceus SRC3 strain as a novel source of the antibiotic adjuvant streptazolin: A statistical approach toward the optimized production. Journal of Microbiological Methods, 148, 161–168. https://doi.org/10.1016/j.mimet.2018.04.00810.1016/j.mimet.2018.04.00829665368 Search in Google Scholar

47. Leulmi, N., Sighel, D., Defant, A., Khenaka, K., Boulahrouf, A., Mancini, I. (2019). Nigericin and grisorixin methyl ester from the Algerian soil–living Streptomyces youssoufiensis SF10 strain: a computational study on their epimeric structures and evaluation of glioblastoma stem cells growth inhibition. Natural Product Research, 33(2), 266–273. https://doi.org/10.1080/14786419.2018.144601410.1080/14786419.2018.144601429513090 Search in Google Scholar

48. Gacem, M. A., Ouled-El-Hadj-Khelil, A., Boudjemaa, B., Wink, J. (2020). Antimicrobial and antioxidant effects of a forest actinobacterium V002 as new producer of spectinabilin, undecylprodigiosin and metacycloprodigiosin. Current Microbiology, 77(10), 2575–2583. https://doi.org/10.1007/s00284-020-02007-110.1007/s00284-020-02007-132372105 Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo