1. bookVolume 13 (2013): Issue 4 (October 2013)
    Issue Editors: Magdalena Bielska, Jerzy Pilawski, Katarzyna Skupniewicz
Journal Details
License
Format
Journal
eISSN
2300-8733
ISSN
1642-3402
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
access type Open Access

The Quality of Porcine Mesenchymal Stem Cells and Their Osteo- and Adipogenic Cell Derivatives – The Level of Proapoptotic Bad Protein Expression / Jakość Mezenchymalnych Komórek Macierzystych Świni Oraz Ich Pochodnych Zróżnicowanych W Kierunku Komórek Szeregu Osteo- I Adipogennego – Poziom Ekspresji Proapoptotycznego Białka Bad

Published Online: 20 Oct 2013
Volume & Issue: Volume 13 (2013) - Issue 4 (October 2013) - Issue Editors: Magdalena Bielska, Jerzy Pilawski, Katarzyna Skupniewicz
Page range: 753 - 763
Journal Details
License
Format
Journal
eISSN
2300-8733
ISSN
1642-3402
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
Abstract

The aim of the research was to evaluate the quality of porcine mesenchymal stem cells (MSCs) and MSC-derived osteoblasts/osteocytes (bone cells) and adipocytes (fat cells). This evaluation was performed on the basis of molecular analysis for proapoptotic BAD protein expression. MSCs isolated from the pig bone marrow were cultured in vitro for five weeks in three types of culture media: differentiating towards the osteoblasts/osteocytes (O) and adipocytes (A) and non-differentiating, control medium (C). In all groups of cells, the relative extent of BAD protein expression was estimated by western blotting. Significant differences in the posttranslational abundance of BAD proteins were noted between MSCs differentiated into the osteogenic and the adipogenic cell lineages (P<0.05). Summarizing the results, we conclude that posttranslational level of BAD protein expression can be used as a reliable marker for assessing the quality of both MSCs and their cell derivatives. Interestingly, the semi-quantitative profile of BAD protein expression in differentiated cells turned out to be lower than that observed in undifferentiated cells, demonstrating that the culture conditions used for pro-osteogenic or pro-adipogenic cellular transformation did not affect negatively the quality of MSCs.

Keywords

Alfaro M.P., Vincent A., Saraswati S., Thorne C.A., Hong C.C., Lee E., Young P.P. (2010). s FRP2 suppression of bone morphogenic protein (BMP) and Wnt signaling mediates mesenchymal stem cell (MSC) self-renewal promoting engraftment and myocardial repair. J. Biol. Chem., 285: 35645-35653.Search in Google Scholar

Antonsson B., Conto F., Ciavatta A.M., Montesuit S., Lewus S., Martinou I., Bernasconi L., Bernard A., Mermod J.C., Mazzei G., Maundrell K., Gambal e F., Sadoul R., Martinou J.C. (1997). Inhibition of Bax channel forming activity by Bcl-2. Science, 277: 330-372.Search in Google Scholar

Ashkenazi A., Dixit V.M. (1998). Death receptors: signaling and modulation. Science, 281: 1305-1308.Search in Google Scholar

Bajek A., Olkowska J., Drewa T. (2011). Mesenchymal stem cells asatherapeutic tool in tissue and organ regeneration (in Polish). Adv. Hyg. Exp. Med., 65: 124-132.Search in Google Scholar

Bieback K., Wuchter P., Besser D., Franke W., Becker M., Ott M., Pacher M., Ma N., Stamm C., Klüter H., Müller A., Ho A.D., START-MSCconsortium (2012). Mesenchymal stromal cells (MSCs): science and f(r)iction. J. Mol. Med. (Berl)., 90: 773-782.Search in Google Scholar

Bosch P., Pratt S.L., Stice S.L. (2006). Isolation, characterization, gene modification, and nuclear reprogramming of porcine mesenchymal stem cells. Biol. Reprod., 74: 46-57.Search in Google Scholar

Cai A., Zheng D., Dong Y., Qiu R., Huang Y., Song Y., Jiang Z., Rao S., Liao X., Kuang J., Dai G., Mai W. (2011). Efficacy of Atorvastatin combined with adipose-derived mesenchymal stem cell transplantation on cardiac function in rats with acute myocardial infarction. Acta. Biochim. Biophys., 43: 857-866.Search in Google Scholar

Caplan A.I. (1991). Mesenchymal stem cells. J. Orthop. Res., 9: 641-650.Search in Google Scholar

Chipuk J.E., Green D.R. (2005). Do inducers of apoptosis trigger caspase-independent cell death? Nat. Rev. Mol. Cell Biol., 6: 268-275.Search in Google Scholar

Coleman C.M., Vaughan E.E., Browe D.C., Mooney E., Howard L., Barry F.P. (2013). Growth differentiation factor-5 enhances in vitro mesenchymal stromal cell chondrogenesis and hypertrophy. Stem Cells Dev., doi:10.1089/scd.2012.0282.10.1089/scd.2012.0282368531623388029Search in Google Scholar

Datta S.R., Dudek H., Tao X., Masters S., Fu H., Gotoh Y., Greenberg M.E. (1997). Akt phosphorylation of BADcouples survival signals to the cell-intrinsic death machinery. Cell, 91: 231-241.Search in Google Scholar

Dominici M., Le Blanc K., Mueller I., Slaper - Cortenbach I., Marini F., Krause D., Deans R., Keating A., Prockop Dj., Horwitz E. (2006). Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 8: 315-317.Search in Google Scholar

Hanada M., Feng J., Hemmings B.A. (2004). Structure, regulation and function of PKB/AKT - a major therapeutic target. Biochim. Biophys. Acta, 1697: 3-16.Search in Google Scholar

Huppeertz B., Frank H.G., Kaufmann P. (1999). The apoptosis cascade morphological and immunohistochemical methods for its visualization. Anat. Embryol., 200: 1-18.Search in Google Scholar

Izadpanah R., Kaushal D., Kriedt C., Tsien F., Patel B., Dufour J., Bunnell B.A. (2008). Long-term in vitro expansion alters the biology of adult mesenchymal stem cells. Cancer Res., 68: 4229-4238.Search in Google Scholar

Juhásová J., Juhás Š., Klíma J., Strnádel J., Holubová M., Motlík J. (2011). Osteogenic differentiation of miniature pig mesenchymal stem cells in 2Dand 3Denvironment. Physiol. Res., 60: 559-571.Search in Google Scholar

Kidd V.J., Lahti J.M., Teitz T. (2000). Proteolytic regulation of apoptosis. Seminars Cell Develop. Biol., 11: 191-201.Search in Google Scholar

Krześlak A. (2010). Akt kinase:akey regulator of metabolism and progression of tumors. Adv. Hyg. Exp. Med., (in Polish)., 64: 490-503.Search in Google Scholar

Laemmli U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.Search in Google Scholar

Mao J.J., Marion N.W. (2009). Essential Stem Cell Methods. Editors Lanza R., Klimanskaya I. Academic Press, 1st ed., 297 pp.Search in Google Scholar

Opiela J., Kątska- Książkiewicz L., Lipiński D., Słomski R., Bzowska M., Ryńska B. (2008 a). Interactions among activity of glucose-6-phosphate dehydrogenase in immature oocytes, expression of apoptosis-related genes Bcl-2 and Bax, and developmental competence following IVPin cattle. Theriogenology, 69: 546-555.10.1016/j.theriogenology.2007.11.00118242680Search in Google Scholar

Opiela J., Kątska - Książkiewicz L., Smorąg Z. (2008 b). DNAanalysis: theory and practice (in Polish). Słomski R. (ed.). University of Life Sciences, Poznań, pp. 530-536.Search in Google Scholar

Quevedo H.C., Hatzistergos K.E., Oskouei B.N., Feigenbaum G.S., Rodri- guez J.E., Valdes D., Pattany P.M., Zambrano J.P., Hu Q., Mc Niece I., Held - man A.W., Hare J.M. (2009). Allogeneic mesenchymal stem cells restore cardiac function in chronic ischemic cardiomyopathy via trilineage differentiating capacity. Proc. Natl. Acad. Sci., 106: 14022-14027.Search in Google Scholar

Ratajczak M.Z., Kucia M. (2005). Stem cells - the challenge of the 21st century? (in Polish). Postępy Biol. Kom., 23: 11-26.Search in Google Scholar

Ringe J., Kaps C., Schmitt B., Büscher K., Bartel J., Smolian H., Schultz O., Bur- mester G.R., Häupl T., Sittinger M. (2002). Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages. Cell Tissue Res., 307: 321-327.Search in Google Scholar

Słupianek A., Pytel D., Majsterek I. (2007). The role of oncogenic tyrosine kinases in the cellular response to anticancer therapy (in Polish). Adv. Hyg. Exp. Med., 61: 819-827.Search in Google Scholar

Stępień A., Izdebska M., Grzanka A. (2007). Types of cell death (in Polish). Adv. Hyg. Exp. Med., 61: 420-428.Search in Google Scholar

Szalata M., Pławski A., Słomski R. (2008). DNAanalysis: theory and practice (in Polish). Słomski R. (ed.). University of Life Sciences, Poznań, pp. 503-511.Search in Google Scholar

Urbaniak - Kujda D., Wołowiec D., Tomaszewska- Toporska B., Kapelko- - Słowik K., Kuliczkowski K. (2005). Mesenchymal stem cells: their biology and prospects for clinical applications. Acta Haematol. Pol., 2: 161-166.Search in Google Scholar

Vaux D.L., Cory S., Adams J.M. (1988). Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-Bcells. Nature, 335: 440-442. Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo