1. bookVolume 56 (2017): Issue 1 (January 2017)
Journal Details
First Published
01 Mar 1961
Publication timeframe
4 times per year
English, Polish
Open Access

Application of the bacterial outer membrane vesicles in vaccine design

Published Online: 21 May 2019
Volume & Issue: Volume 56 (2017) - Issue 1 (January 2017)
Page range: 43 - 55
Received: 01 Jun 2016
Accepted: 01 Sep 2016
Journal Details
First Published
01 Mar 1961
Publication timeframe
4 times per year
English, Polish

Outer membrane vesicles (OMVs) are extracellular structures produced by most gram-negative bacteria, including pathogens of humans and animals. OMVs play an important role in the physiology of microorganisms and are an integral part of many biological processes. Following the discovery that they are able to transport many biomolecules, also these which have the ability to interact with the immune system, their potential use as non-replicating vaccines has become an important aspect of immunotherapeutic researches. These nano-sized elements exhibit remarkable potential for immunomodulation of immune response, thanks to the ability to deliver naturally or artificially incorporated antigens within their structure. First vaccine based on outer membrane vesicles was developed almost 30 years ago against Neisseria meningitidis serogroup B. This review presents some basic information on biogenesis and functions of OMVs. It also provides examples of pathogens, whose OMVs (in natural or modified form) have been used in the development of immunogenic vaccines against the organisms from which the vesicles had been obtained. OMVs are proving to be more versatile than first conceived and may become important part of biotechnology research, not limited to medical applications.

1. Introduction. 2. Outer membrane vesicles biogenesis. 3. Biological functions of outer membrane vesicles. 3.1. Role in response to stressors. 3.2. Role in the extracellular transport. 3.3. Role in biofilm formation. 4. OMVs in vaccine construction. 4.1. Neisseria meningitidis. 4.2. Vibrio cholerae. 4.3. Bordetella pertussis. 4.4. Chlamydia trachomatis. 4.5. Burkholderia pseudomallei. 4.6. Acinetobacter baumannii. 4.7. Francisella noatunensis. 4.8. Shigella spp. 4.9. Campylobacter jejuni. 5. Conclusions

1. Wprowadzenie. 2. Biogeneza pęcherzyków zewnątrzbłonowych. 3. Funkcje pęcherzyków zewnątrzkomórkowych. 3.1. Udział w odpowiedzi na czynniki stresogenne. 3.2. Udział w transporcie pozakomórkowym. 3.3. Udział w tworzeniu biofilmu. 4. Pęcherzyki zewnątrzbłonowe w konstrukcji szczepionek. 4.1. Neisseria meningitidis. 4.2. Vibrio cholerae. 4.3. Bordetella pertussis. 4.4. Chlamydia trachomatis. 4.5. Burkholderia pseudomallei. 4.6. Acineto bacter baumannii. 4.7. Francisella noatunensis. 4.8. Shigella spp. 4.9. Campylobacter jejuni. 5. Podsumowanie

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