1. bookVolume 63 (2014): Issue 1-6 (December 2014)
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eISSN
2509-8934
First Published
22 Feb 2016
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Open Access

Short note: Development, characterization and cross-amplification of eight EST-derived microsatellites in Salix

Published Online: 01 Jun 2017
Volume & Issue: Volume 63 (2014) - Issue 1-6 (December 2014)
Page range: 113 - 115
Received: 04 Nov 2013
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

A set of eight simple sequence repeat (SSR) markers were developed from 707 Salix expressed sequence tags (ESTs) deposited in GenBank. Each of the EST-SSR amplicons was identical to the original EST, with sequence identity 60.90-96.03% and presence of the expected repeat motifs. Of the eight EST-SSR loci, five were polymorphic among 14 individuals of S. eriocephala, with the number of alleles per locus (Na), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content (PIC) being 2-7 (mean 4.8), 0.29-0.85 (mean 0.65), 0.25-0.84 (mean 0.65) and 0.21-0.78 (mean 0.58), respectively. High rates of crossspecies/ genus amplification were also observed within fourteen different species. The primer sequences for the eight EST-SSRs have been deposited in the Probe database of GenBank (IDs Pr031820546 - Pr031820553). The EST-SSRs developed herein would be a valuable addition of functional markers for genetics and breeding applications in a wide range of Salix species.

Keywords

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