1. bookVolume 58 (2009): Issue 1-6 (December 2009)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Distribution of Microtubular Cytoskeletons and Organelle Nucleoids During Microsporogenesis in a 2n Pollen Producer of Hybrid Populus

Published Online: 19 Oct 2017
Volume & Issue: Volume 58 (2009) - Issue 1-6 (December 2009)
Page range: 220 - 226
Received: 02 Aug 2008
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

The distribution of microtubular cytoskeletons and organelle nucleoids during microsporogenesis in Populus simonii Carr. x P. nigra L. ‘Tongliao’ was studied by indirect immunofluorescence and 4’,6-diamidino-2- phenylindole (DAPI) staining. Our purpose was to reveal the relationship between changes in microtubule and chromosome behavior and cytokinesis and to explain the mechanism of 2n pollen production. We observed stable frequencies of 2n pollen production, ranging from 1.56% to 2.29%, between 2006 and 2008. Moreover, conjoined pollen grains were also observed. Meiotic abnormalities, including univalents, lagging chromosomes and micronuclei, were observed during microsporogenesis. Triads and dyads were also detected in meiotic products. Parallel spindles in metaphase II were unable to fuse, owing to the existence of an organelle band. Regularly, in telophase II, primary phragmoplasts were organized between sister nuclei, and secondary phragmoplasts formed between non-sister nuclei. Cell plates were initiated by centrifugal expansion of phragmoplasts and cytoplasmic infurrowing started at the junctions between the microsporocyte wall and the expanded phragmoplasts. However, a secondary phragmoplast was absent in some microsporocytes. These observations suggest that the occurrence of 2n pollen may result from the partial failure of cytokinesis caused by the absence of secondary phragmoplasts in this hybrid.

Keywords

BROWN, R. C. and B. E. LEMMON (1988a): Sporogenesis in bryophytes. Adv Bryolo 3: 159-223. Search in Google Scholar

BROWN, R. C. and B. E. LEMMON (1988b): Preprophasic microtubule systems and development of the mitotic spindle in hornworts. Protoplasma 143: 11-21. 10.1007/BF01282954Search in Google Scholar

BROWN, R. C. and B. E. LEMMON (1988c): Microtubules associated with simultaneous cytokinesis of coenocytic microsporocytes. Amer J Bot 75(12): 1848-1856. 10.1002/j.1537-2197.1988.tb11265.xSearch in Google Scholar

BROWN, R. C. and B. E. LEMMON (1989): Minispindles and cytoplasmic domains in microsporogenesis of orchids. Protoplasma 148: 26-32. 10.1007/BF01403988Search in Google Scholar

BROWN, R. C. and B. E. LEMMON (1991a): Pollen development in orchids. 1. Cytoskeleton and the control of division plane in irregular patterns of cytokinesis. Protoplasma 163: 9-18. 10.1007/BF01323402Search in Google Scholar

BROWN, R. C. and B. E. LEMMON (1991b): Pollen development in orchids. 2. The cytokinetic apparatus in simultaneous cytokinesis. Protoplasma 165: 155-166. 10.1007/BF01322286Open DOISearch in Google Scholar

BROWN, R. C. and B. E. LEMMON (1992): Control of division plane in normal and griseofulvin-treated microsporocytes of Magnolia. J Cell Sci 103: 1031-1038. 10.1242/jcs.103.4.1031Search in Google Scholar

BROWN, R. C. and B. E. LEMMON (1996): Nuclear cytoplasmic domains, microtubules and organelles in microsporocytes of the slipper orchid Cypripedium californicum A. Gray dividing by simultaneous cytokinesis. Sex Plant Reprod 9: 145-152. 10.1007/BF02221394Open DOISearch in Google Scholar

CAI, X. (1999): The dynamics and significance of cytoskeletons in pollen formation of angiosperms. Chin Bull Bot 16(4): 339-344. Search in Google Scholar

CONICELLA, C., A. CAPO, M. CAMMARERI, A. ERRICO, N. SHAMINA and L. M. MONTI (2003): Elucidation of meiotic nuclear restitution mechanisms in potato through analysis of microtubular cytoskeleton. Euphytica 133: 107-115. 10.1023/A:1025636321757Search in Google Scholar

DICKINSON, H. G. (1981): The structure and chemistry of plastid differentiation during male meiosis in Lilium henryi. J Cell Sci 52: 223-241.10.1242/jcs.52.1.2236174533Search in Google Scholar

GUNNING, B. E. S. (1982): The cytokinetic apparatus: its development and spatial regulation, pp. 229-292. In: The cytoskeleton in plant growth and development, edited by C. W. LLOYD, Academic Press, London. Search in Google Scholar

GUNNING, B. E. S., A. R. HARDHAM and J. E. HUGHES (1978): Preprophase bands in all categories of formative and proliferative cell division in Azolla roots. Planta 143: 145-160.10.1007/BF0038778624408365Search in Google Scholar

JANSSON, S. and C. J. DOUGLAS (2007): Populus: A model system for plant biology. Annu Rev Plant Biol 58: 435-458. 10.1146/annurev.arplant.58.032806.10395617280524Open DOISearch in Google Scholar

KANG, X. Y. (2002): Mechanism of 2n pollen occurring in Chinese white poplar. J Beijing For Univ 24(5/6): 67-70. Search in Google Scholar

KLECKNER, N. (1996): Meiosis: How could it work? Proc Natl Acad Sci USA 93: 8167-8174. 10.1073/pnas.93.16.8167386418710842Open DOISearch in Google Scholar

MASHKINA, O. S., L. M. BURDAEVA, M. M. BELOZEROVA and L. N. VYUNOVA (1989): Method of obtaining diploid pollen of woody species. Lesovedenie 1: 19-25. Search in Google Scholar

MOK, D. W. and S. J. PELOQUIN (1975): Three mechanisms of 2n pollen formation in diploid potatoes. Can J Genet Cytol 17: 217-225.10.1139/g75-029Open DOISearch in Google Scholar

PFEIFFER, T. W. and E. T. BINGHAM (1983): Abnormal meiosis in alfalfa, Medicago sativa: cytology of 2n egg and 4n pollen formation. Can J Genet Cytol 25: 107-112. 10.1139/g83-021Open DOISearch in Google Scholar

RAE, A. M., N. R. STREET and M. RODRÍGUEZ-ACOSTA (2007): Populus trees, pp. 1-28. In: Genome mapping and molecular breeding in plants, vol. 7, Forest trees, edited by C. KOLE, Springer-Verlag, Berlin, Heidelberg. 10.1007/978-3-540-34541-1_1Search in Google Scholar

RAMANNA, M. S. (1974): The origin of unreduced microspores due to aberrant cytokinesis in the meiocytes of potato and its genetic significance. Euphytica 23: 20-30. 10.1007/BF00032736Open DOISearch in Google Scholar

RAMANNA, M. S. (1979): A re-examination of the mechanisms of 2n gamete formation in potato and its implications for breeding. Euphytica 28: 537-561. 10.1007/BF00038921Open DOISearch in Google Scholar

RAMSEY, J. and D. W. SCHEMSKE (1998): Pathways, mechanisms, and rates of polyploidy formation in flowering plants. Annu Rev Ecol Syst 29: 467-501. 10.1146/annurev.ecolsys.29.1.467Open DOISearch in Google Scholar

RAY, M. and M. K. TOKACH (1992): Cytology of 2n pollen formation in diploid crested wheatgrass, Agropyron cristatum. Crop Sci 32: 1361-1365.10.2135/cropsci1992.0011183X003200060011xSearch in Google Scholar

RHEE, S. Y. and C. R. SOMERVILLE (1998): Tetrad pollen formation in quartet mutants of Arabidopsis thaliana is associated with persistence of pectic polysaccharides of the pollen mother cell wall. Plant J 15(1): 79-88.10.1046/j.1365-313X.1998.00183.x9744097Open DOISearch in Google Scholar

SCHOPFER, C. and P. HEPLER (1991): Distribution of Membranes and the Cytoskeleton During Cell Plate Formation in Pollen Mother Cells of Tradescantia. J Cell Sci 100: 717-728. 10.1242/jcs.100.4.717Search in Google Scholar

SHAMINA, N. V., E. I. GORDEEVA, N. M. KOVALEVA, E. G. SERIUKOVA and N. V. DOROGOVA (2007): Formation and function of phragmoplast during successive cytokinesis stages in higher plant meiosis. Cell Biol Int 31: 1-10. 10.1016/j.cellbi.2006.12.00117291791Open DOISearch in Google Scholar

SHELDON, J. M. and H. G. DICKINSON (1986): Pollen wall formation in Lilium: The effect of chaotropic agents, and the organisation of the microtubular cytoskeleton during pattern development. Planta 168: 11-23.10.1007/BF0040700324233729Search in Google Scholar

SMITH, E. C. (1943): A study of cytology and speciation in the genus Populus L. J Arnold Arbor, Harvard University 24(3): 275-305.10.5962/p.185479Search in Google Scholar

TAKANA, I. (1991): Microtubule-determined plastid distribution during microsporogenesis in Lilium longiflorum. J Cell Sci 99: 21-31. 10.1242/jcs.99.1.21Search in Google Scholar

WANG, J., X. Y. KANG, D. L. LI, Y. C. JING and Z. H. ZHANG (2006): Meiosis and chromosome behavior of pollen mother cell in Populus simonii Carr. x P. nigra L. ‘Tongliao’. Acta Bot Boreal-Occident Sin 26(11): 2231-2238.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo