1. bookVolume 61 (2015): Issue 4 (December 2015)
Journal Details
First Published
04 Apr 2014
Publication timeframe
4 times per year
access type Open Access

Incubation methods for the detection of fungi associated with caraway (Carum carvi L.) seeds

Published Online: 06 Jan 2016
Volume & Issue: Volume 61 (2015) - Issue 4 (December 2015)
Page range: 9 - 22
Journal Details
First Published
04 Apr 2014
Publication timeframe
4 times per year

Introduction: Infestation with fungi may significantly affect the quality of seeds. However, there is no standard method for caraway (Carum carvi L.) seed health testing.

Objective: The aim of the present study was to determine the most efficient method of the detection of fungi associatyed with caraway seeds.

Methods: Seven incubation methods for evaluation of health of these seeds were compared: deep freeze blotter test, blotter test with mannitol, blotter test with polyethylene glycol, agar tests on potato-dextrose-agar (PDA) and on reduced PDA (RPDA) without seed disinfection, and agar tests on PDA and RPDA after seed disinfection. The evaluation was performed after 10 and 14 days of incubation.

Results: Thirty two fungal genera were associated with the seeds. Alternaria alternata, Cladosporium spp. and Rhizopus nigricans were identified most frequently. Prolongation of incubation time favoured growth of Fusarium spp. and R. nigricans to the highest extent.

Conclusions: The greatest seed infestation with fungi, especially Alternaria spp., was observed in the deep freeze blotter test followed by the blotter test with mannitol. Both of them could be recommended for further study on caraway seed health testing.


Słowa kluczowe:

1. Sedláková J, Kocurková B, Kubáň V. Determination of essential oils content and composition in caraway (Carum carvi L.). Czech J Food Sci 2001; 19(1):31-6.10.17221/6571-CJFSSearch in Google Scholar

2. Simic A, Rančic A, Sokovic MD, Ristic M, Grujic-Jovanovic S, Vukojevic J et al. Essential oil composition of Cymbopogon winterianus and Carum carvi and their antimicrobial activities. Pharm Biol 2008; 46(6):437-41.10.1080/13880200802055917Search in Google Scholar

3. Seidler-Łożykowska K, Kędzia B, Karpińska E, Bocianowski J. Microbiological activity of caraway (Carum carvi L.) essential oil obtained from different origin. Acta Sci Agron 2013; 35(4), doi: 10.4025/actasciagron.v35i4.16900.10.4025/actasciagron.v35i4.16900Search in Google Scholar

4. Hartmans KJ, Diepenhorst P, Bakker W, Gorris LGM. The use of carvone in agriculture: sprout suppression of potatoes and antifungal activity against potato tuber and other plant diseases. Ind Crop Prod 1995; 4(1):3-13.10.1016/0926-6690(95)00005-WSearch in Google Scholar

5. Iacobellis NS, Lo Cantore P, Capasso F, Senatore F. Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils. J Agric Food Chem 2005; 53(1):57-61.10.1021/jf048735115631509Search in Google Scholar

6. Zheng GQ, Kenney PM, Lam LKT. Anethofuran, carvone, and limonene: potential cancer chemoprotective agents from dill weed oil and caraway oil. Planta Med 1992; 58(4):338-41.10.1055/s-2006-9614801438594Search in Google Scholar

7. Kamaleeswari M, Deeptha K, Sengottuvelan M, Nalini N. Effect of dietary caraway (Carum carvi L.) on aberrant crypt foci development, fecal steroids, and intestinal alkaline phosphatase activities in 1,2-dimethylhydrazine-induced colon carcinogenesis. Toxicol Appl Pharmacol 2006; 214(3):290-6.10.1016/j.taap.2006.01.00116483625Search in Google Scholar

8. Kamaleeswari M, Nalini N. Dose-response efficacy of caraway (Carum carvi L.) on tissue lipid peroxidation and antioxidant profile in rat colon carcinogenesis. J Pharm Pharmacol 2006; 58(8):1121-30.10.1211/jpp.58.8.001416872560Search in Google Scholar

9. Eddouks M, Lemhadri A, Michel JB. Caraway and caper: potential anti-hyperglycaemic plants in diabetic rats. J Ethnopharmacol 2004; 94(1):143-8.10.1016/j.jep.2004.05.00615261975Search in Google Scholar

10. Lahlou S, Tahraoui A, Israili Z, Lyoussi B. Diuretic activity of the aqueous extracts of Carum carvi and Tanacetum vulgare in normal rats. J Ethnopharmacol 2007; 110(3):458-63.10.1016/j.jep.2006.10.00517113735Search in Google Scholar

11. Samojlik I, Lakić N, Mimica-Dukić N, Đaković-Švajcer K, Božin B. Antioxidant and hepatoprotective potential of essential oils of coriander (Coriandrum sativum L.) and caraway (Carum carvi L.) (Apiaceae). J Agric Food Chem 2010; 58(15):8848-53.10.1021/jf101645n20608729Search in Google Scholar

12. Carvalho CCCR, Fonseca MMR. Carvone: Why and how should one bother to produce this terpene. Food Chem 2006; 95:413-422.10.1016/j.foodchem.2005.01.003Search in Google Scholar

13. Machowicz-Stefaniak Z, Zalewska E. Biodiversity of fungi colonizing different parts of caraway (Carum carvi L.), EJPAU 2008; 11 (1):#21, http://www.ejpau.media.pl/volume11/issue1/art-21.htmlSearch in Google Scholar

14. Machowicz-Stefaniak Z, Zimowska B. Grzyby przenoszone przez materiał siewny roślin zielarskich. Acta Agrob 2000; 53(2):25-38.Search in Google Scholar

15. Mačkinaitė R. Fungi diversity on wild and cultivated common caraway (Carum carvi L.) seeds. Žemdirbystė – Agriculture 2010; 97(4):73-84.Search in Google Scholar

16. Mačkinaitė R. Internal mycobiota of wild and cultivated common caraway (Carum carvi L.) seeds. Žemdirbystė – Agriculture 2011; 98(2):183-94.Search in Google Scholar

17. Mačkinaitė R. Potential pathogens of common caraway (Carum carvi L.) seeds and search for measures suppressing their spread. Žemdirbystė – Agriculture 2012; 99(2):179-88.Search in Google Scholar

18. Mazur S, Nawrocki J. Fungal diseases threat on caraway plantations in the south region of Poland. Acta Fyto Zoot 2004; 7:201-3.Search in Google Scholar

19. Odstrčilova L. Changes in the occurrence of mycoflora on caraway seeds after fungicide application. Plant Protect Sci 2007; 43:146-50.10.17221/2240-PPSSearch in Google Scholar

20. Machowicz-Stefaniak Z, Zalewska E. Occurrence of Colletotrichum dematium on selected herbs species and preparations inhibiting pathogen’s growth and development in vitro. Ecol Chem Eng S 2011; 18(4):465-78.Search in Google Scholar

21. Machowicz-Stefaniak Z, Zalewska E, Król E. Occurrence, harmfulness and morphological structures of Colletotrichum gleosporioides (Penz.) Sacc. (teleomorph: Glomerella cingulata (Stonem.) Spauld. Et Schrenk). Acta Sci Pol, Hortorum Cultus 2011; 10(3):39-52.Search in Google Scholar

22. Zalewska E. Pathogenicity of Colletotrichum dematium (Fr.) Grove to caraway Carum carvi L. Acta Agrobot 2010; 63(1):137-47.10.5586/aa.2010.016Search in Google Scholar

23. Reuveni R. Fusarium equiseti – a new cause of cumin spice plant wilt in Israel. Plant Disease 1982; 66(6):498-499.10.1094/PD-66-498Search in Google Scholar

24. Logrieco A, Moretti A, Solfrizzo M. Alternaria toxins and plant diseases: an overview of origin, occurrence and risks. World Mycotoxin J 2009; 2(2):129-140.10.3920/WMJ2009.1145Search in Google Scholar

25. Regina M, Rama T. Biochemical changes in stored caraway seeds due to fungi. Indian Phytopathol 1992; 45(3):384.Search in Google Scholar

26. Cappelli C, Covarelli L, Methods used in seed pathology and their recent improvements. Phytopathol Pol 2005; 35:11-18.Search in Google Scholar

27. ISTA. International Rules for Seed Testing. Annexe to Chapter 7 Seed Health Testing, Seed Health Testing Methods. The International Seed Testing Association, Bassersdorf, Switzerland 2012.Search in Google Scholar

28. Machado JC, Cautinho WM, Guimarães RM, Vieira MGGC, Ferreira DF. Use of osmotic solutes to control seed germination of rice and common bean in seed health blotter tests. Seed Sci Technol 2008; 36(1):66-75.10.15258/sst.2008.36.1.07Search in Google Scholar

29. Machado JC, Guimaraes RM, Vieira MGGC, Souza RM, Pozza EA. Use of water restriction technique in seed pathology. Seed Testing International 2004; 128:14-18.Search in Google Scholar

30. Machado JC, Oliveira JA, Vieira MGGC, Alves MC. Control of soybeen seeds germination in seed health tests using water restriction technique. On-line. Rev Bras Sementes 2003; 25(2). doi: http://dx.doi.org/10.1590/S0101-31222003000400011.Search in Google Scholar

31. Rosińska A, Jarosz M, Szopińska D, Dorna H, Tylkowska K. Comparison of methods for detecting fungi in Silybum marianum (L.) Gaertn. seeds. Folia Hort 2013; 25(2):107-115. doi: http://dx.doi.org/10.2478/fhort-2013-0012.Search in Google Scholar

32. Sauer DB, Burroughs R. Disinfection of seed surfaces with sodium hypochlorite. Phytopathology 1986; 76:745-749.10.1094/Phyto-76-745Search in Google Scholar

33. Swagel EN, Van H Bernhard A, Ellmore GS. Substrate water potential constraints on germination of the strangle fig Ficus aurea (Moraceae). Am J Bot 1997; 84:716-722.10.2307/2445908Search in Google Scholar

34. Michel BE, Kaufmann MR. The osmotic potential of polyethylene glycol 6000. Plant Physiol 1973; 51:914-916.10.1104/pp.51.5.91436637516658439Search in Google Scholar

35. Ellis MB. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute Kew, England 1971.Search in Google Scholar

36. Ellis MB. More Dematiaceous Hyphomycetes. Commonwealth Mycological Institute Kew, England 1976.Search in Google Scholar

37. Machado JC, Langerak CJ, Jaccoud-Filho DS. Seed-borne fungi: A contribution to routine seed health analysis. International Seed Testing Association. Bassersdorf 2002.Search in Google Scholar

38. Mathur SB, Kongsdal O. Common laboratory seed health testing methods for detecting fungi. International Seed Testing Association, Bassersdorf, Switzerland 2003.Search in Google Scholar

39. Tischler W. Grundzüge der terrestrischen Tierökologie. Friedrich Vieweg und Sohn, Braunschweig, Germany1949.10.1007/978-3-663-02549-8Search in Google Scholar

40. Szopińska D, Tylkowska K, Deng ChJ, Gao Y. Comparison of modified blotter and agar incubation methods for detecting fungi in Zinnia elegans seeds. Seed Sci Technol 2012; 40:32-42.10.15258/sst.2012.40.1.04Search in Google Scholar

41. Woody MA, Chu FS. Toxicology of Alternaria mycotoxins. In: Chełkowski J, Visconti A, eds. Alternaria biology, plant diseases and metabolites. Amsterdam, Elsevier, 1992:409-434.Search in Google Scholar

42. Bulajić A, Djekić I, Lakić N, Krstić B. The presence of Alternaria spp. on the seed of Apiaceae plants and their influence on seed emergence. Arch Biol Sci 2009; 61(4):871-881.10.2298/ABS0904871BSearch in Google Scholar

43. ISTA. International Rules for Seed Testing. Annexe to Chapter 7: Seed Health Testing Methods. 7-001a: Blotter method for the detection of Alternaria dauci on Daucus carota. ISTA, Bassersdorf, Switzerland 2014a.Search in Google Scholar

44. ISTA. International Rules for Seed Testing. Annexe to Chapter 7: Seed Health Testing Methods. 7-001b: Blotter method for the detection of Alternaria radicina on Daucus carota. ISTA, Bassersdorf, Switzerland 2014b.Search in Google Scholar

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