1. bookVolume 16 (2016): Issue 2 (April 2016)
Journal Details
License
Format
Journal
eISSN
2300-8733
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
access type Open Access

7. Associations between Polymorphisms in the DIO3 Gene and Reproductive Traits and Carcass Performance in Pigs

Published Online: 06 May 2016
Volume & Issue: Volume 16 (2016) - Issue 2 (April 2016)
Page range: 399 - 413
Received: 17 Apr 2015
Accepted: 26 Oct 2015
Journal Details
License
Format
Journal
eISSN
2300-8733
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
Abstract

Recently, DIO3 gene has been proposed as a candidate gene for litter size in pigs. Moreover, it was shown that polymorphism in this gene is associated with carcass traits. In this study we identified several SNPs within coding sequence of DIO3 by HRM method and performed association study between two polymorphisms and reproductive and carcass traits in pigs bred in Poland. Analysis of 350 pigs of Landrace and Large White breed revealed several significant associations for rs80999359, like period between the second and third parities (2IP)(P<0.0008) in the whole population, period between the third and fourth parities (3IP) (P<0.022), number of piglets born alive (L3NBA) (P<0.0084) and number of piglets at 21 days (L3NB21d) (P<0.0176) at the third parity in Large White as well as period between the second and third parities (2IP) (P<0.0012) in Landrace breed. The second polymorphism (rs80983654) was associated with 1IP (P<0.0218), number of piglets born alive at the fourth parity (L4NBA, P<0.027), number of piglets at 21 day at the fourth litter (L4NB21d, P<0.01), in the whole population, average number of piglets born alive (ANBA, P<0.01250), average number of piglets at 21 day (ANB21d, P<0.009), average interparity period (AIP, P<0.016), age at the first parity (1AP, P<0.003), (1IP, P<0.001, L4NBA, P<0.017, L4NB21d, P<0.005) in Large White breed. In contrast, we have found only few associations between DIO3 polymorphisms and carcass traits. rs80999359 was associated with backfat thickness (p<0.01) while rs80983654 with the weight of ham. Our results suggest that polymorphisms within DIO3 gene may be associated with reproductive traits.

Keywords

Alfonso L. (2005). Use of meta-analysis to combine candidate gene association studies: application to study the relationship between the ESR Pvu IIpolymorphism and sow litter size. Genet. Sel. Evol., 37: 1-19.Search in Google Scholar

Charalambous M., Hernandez A. (2013). Genomic imprinting of the type 3 thyroid hormone deiodinase gene: Regulation and developmental implications. Biochim. Biophys. Acta., 1830: 3946-3955.Search in Google Scholar

Cockett N.E., Jackson S.P., Shay T.L., Farnir F., Berghmans S., Snowder G., Nielsen D.M., Georges M. (1996). Polar overdominance at the ovine callipyge locus. Science, 273: 236-238.Search in Google Scholar

Coster A., Madsen O., Heuven H.C.M., Dibbits B., Groenen M.A.M.,van Aren - donk J.A.M., Bovenhuis H. (2012). The imprinted Gene DIO3 isacandidate gene for litter size in pigs. PLo S ONE, 7: e31825.Search in Google Scholar

Distl O. (2007). Mechanisms of regulation of litter size in pigs on the genome level. Reprod. Domest. Anim., 42: 10-16.Search in Google Scholar

Hernandez A. (2005). Structure and function of the type 3 deiodinase gene. Thyroid, 15: 865-874.Search in Google Scholar

Hernandez A., Martinez M.E., Fiering S., Galton V.A., St Germain D. (2006). Type 3 deiodinase is critical for the maturation and function of the thyroid axis. J. Clin. Invest., 116: 476-484.Search in Google Scholar

Kester M.H., Toussaint M.J., Punt C.A., Matondo R., Aarnio A.M., Darras V.M., Everts M.E.,de Bruin A., Visser T.J. (2009). Large induction of type IIIdeiodinase expression after partial hepatectomy in the regenerating mouse and rat liver. Endocrinology, 150: 540-545.Search in Google Scholar

Magee D.A., Berry D.P., Berkowicz E.W., Sikora K.M., Howard D.J., Mullen M.P., Evans R.D., Spillane C., Mac Hugh D.E. (2011). Single nucleotide polymorphisms within the bovine DLK1-DIO3 imprinted domain are associated with economically important production traits in cattle. J. Hered., 102: 94-101.Search in Google Scholar

Oczkowicz M., Mucha A., Tyra M., Ropka- Molik K., Piórkowska K. (2013). Lack of the associations of the polymorphisms in IGF2, MC4Rand GNASgenes with reproduction traits in pigs and imprinting analysis of IGF2 gene in ovary and cornus uteri. Reprod. Domest. Anim., 48: 562-568.Search in Google Scholar

Qiao M., Wu H-Y., Guo L., Mei SQ., Zhang P-P., Li F-E., Zheng R., Deng C-Y. (2012). Imprinting analysis of porcine DIO3 gene in two fetal stages and association with carcass and meat quality traits. Mol. Biol. Rep., 39: 2329-2335.Search in Google Scholar

Tsai C.E., Lin S.P., Ito M., Takagi N., Takada S., Ferguson-Smith A.C. (2002). Genomic imprinting contributes to thyroid hormone metabolism in the mouse embryo. Curr. Biol., 12: 1221-1226.Search in Google Scholar

Yang Z.L., Cheng H.C., Xia Q.Y., Jiang C.D., Deng C.Y., Li Y.M. (2009). Imprinting analysis of RTL1 and DIO3 genes and their association with carcass traits in pigs (Sus scrofa). Agri. Sci. China, 8: 613-619.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo