1. bookVolume 67 (2017): Issue 2 (June 2017)
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Open Access

Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)

Published Online: 26 Jun 2017
Volume & Issue: Volume 67 (2017) - Issue 2 (June 2017)
Page range: 285 - 291
Received: 11 Aug 2016
Accepted: 22 Feb 2017
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

Gender identification of fish species is carried out mainly by examining external morphological characteristics, which in general, it is very complex and not always a reliable approach. Electrophoresis of plasma proteins can be used as an alternative and useful molecular tool for a more precise sex determination. The presence of female specific proteins in the plasma is a starting point for the application of this technique. In this study, reducing discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze plasma proteins of male and female koi carp (Cyprinus carpio haematopterus). Image analyses of electrophoregrams with resolved plasma proteins by SDS-PAGE showed that it is an appropriate technique to discriminate male from female samples. It is based on the presence of apolipoprotein B-100 which can be used as a suitable marker. Further amino acid characterization of apolipoprotein B-100 confirmed that it is a specific protein for female individuals.

Keywords

1. Surinova S, Schiess R, Huttenhain R, Cerciello F, Wollscheid B, Aebersold R: On the Development of Plasma Protein Biomarkers. J Proteome Res 2011, 10:5-16.10.1021/pr1008515Search in Google Scholar

2. Yilmaz M, Yilmaz R, Alas A: An electrophoretic taxonomic study on serum proteins of Acanthobrama marmid, Leuciscus cephalus, and Chondrostoma regium. EurAsia J BioSci 2007, 3:22-27.Search in Google Scholar

3. Kumar LK, Gopalakrishnan A, Mohindra V, James PB: Identification of female specific protein in seabass, Lates calcarifer (Bloch). Ind J Fish 1999, 46:375-381.Search in Google Scholar

4. Babin PJ: Apolipoproteins and the association of egg yolk proteins with plasma high density lipoproteins after ovulation and follicular atresia in the rainbow trout (Salmo gairdneri). J Biol Chem 1987, 262:4290-4296.10.1016/S0021-9258(18)61346-8Search in Google Scholar

5. Zhang H, Alvin Y L, Loriaux P, Wollscheid B, Zhou Y, Watts J, Aebersold R: Mass spectrometric detection of tissue proteins in plasma. Mol. Cell. Proteomics 2007, 6-1:64-7110.1074/mcp.M600160-MCP20017030953Search in Google Scholar

6. Holmes K, Pitham T, Fletcher N: The world of Koi. Barron’s Educational Series, Inc., Hauppauge, NY, 2005Search in Google Scholar

7. Zapata I, Zerby H, Wick M:. Functional proteomic analysis predicts beef tenderness and the tenderness differential. J Agri Food Chem 2009, 57:4956-496310.1021/jf900041j19449808Search in Google Scholar

8. Theophilus J, Rao PR: Electrophoretic studies on the plasma proteins of the three species of genus channa. Ind. J. Fish 1998, 35:294-297.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo